ArticleJournal of virology2003
Caspase cleavage of the nonstructural protein NS1 mediates replication of Aleutian mink disease parvovirus.
Article in Journal of virology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 58 citations in OpenAlex.
- Viral communities and identification of a parvovirus and two picornaviruses in geese with gout.The Journal of veterinary medical science · 2026Article
- Chicken caspase-3 promotes IBDV replication via the cleavage of IRF7.Frontiers in microbiology · 2026Article
- Host proteases: key regulators in viral infection and therapeutic targeting.Frontiers in immunology · 2025Review
- AMDV Vaccine: Challenges and Perspectives.Viruses · 2021Review
- RNA-seq transcriptome analysis in flounder cells to compare innate immune responses to low- and high-virulence viral hemorrhagic septicemia virus.Archives of virology · 2021Article
- Caspase-mediated cleavage of nucleocapsid protein of a protease-independent porcine epidemic diarrhea virus strain.Virus research · 2020Article
- Molecular epidemiology of chicken anaemia virus in sick chickens in China from 2014 to 2015.PloS one · 2019Article
- Article
- Viral hijacking of host caspases: an emerging category of pathogen-host interactions.Cell death and differentiation · 2017Review
- Outbreak tracking of Aleutian mink disease virus (AMDV) using partial NS1 gene sequencing.Virology journal · 2017Article
- Genetic characterization of the complete genome of an Aleutian mink disease virus isolated in north China.Virus genes · 2016Article
- During Infection, Theiler's Virions Are Cleaved by Caspases and Disassembled into Pentamers.Journal of virology · 2016Article
- Amdoparvoviruses in small mammals: expanding our understanding of parvovirus diversity, distribution, and pathology.Frontiers in microbiology · 2015Review
- Avian reovirus-triggered apoptosis enhances both virus spread and the processing of the viral nonstructural muNS protein.Virology · 2014Article
- Molecular characterization of the small nonstructural proteins of parvovirus Aleutian mink disease virus (AMDV) during infection.Virology · 2014Article
- Structure of the NS1 protein N-terminal origin recognition/nickase domain from the emerging human bocavirus.Journal of virology · 2013Article
- Epstein-Barr virus-encoded microRNA BART15-3p promotes cell apoptosis partially by targeting BRUCE.Journal of virology · 2013Article
- Cleavage of the Junin virus nucleoprotein serves a decoy function to inhibit the induction of apoptosis during infection.Journal of virology · 2013Article
- Article
- Molecular epidemiology of Aleutian disease virus in free-ranging domestic, hybrid, and wild mink.Evolutionary applications · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Virus-induced apoptosis of infected cells can limit both the time and the cellular machinery available for virus replication. Hence, many viruses have evolved strategies to specifically inhibit apoptosis. However, Aleutian mink disease parvovirus (ADV) is the first example of a DNA virus that not only induces apoptosis but also utilizes caspase activity to facilitate virus replication. To determine the function of caspase activity during ADV replication, virus-infected cell lysates or purified ADV proteins were incubated with various purified caspases. Caspases cleaved the major nonstructural protein of ADV (NS1) at two caspase recognition sequences, whereas ADV structural proteins could not be cleaved. Importantly, the NS1 products could be identified in ADV-infected cells but were not present in infected cells pretreated with caspase inhibitors. By mutating putative caspase cleavage sites (D to E), we mapped the two cleavage sites to amino acid residues NS1:227 (INTD downward arrow S) and NS1:285 (DQTD downward arrow S). Replication of ADV containing either of these mutations was reduced 10(3)- to 10(4)-fold compared to that of wild-type virus, and a construct containing both mutations was replication defective. Immunofluorescent studies revealed that cleavage was required for nuclear localization of NS1. The requirement for caspase activity during permissive replication suggests that limitation of caspase activation and apoptosis in vivo may be a novel approach to restricting virus replication.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.