ArticleNaunyn-Schmiedeberg's archives of pharmacology2003
Inhibition of erythrocyte cation channels and apoptosis by ethylisopropylamiloride.
Article in Naunyn-Schmiedeberg's archives of pharmacology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 74 citations in OpenAlex.
- Ion Transport in Eryptosis, the Suicidal Death of Erythrocytes.Frontiers in cell and developmental biology · 2020Review
- Eryptosis: An Erythrocyte's Suicidal Type of Cell Death.BioMed research international · 2018Review
- Triggers, inhibitors, mechanisms, and significance of eryptosis: the suicidal erythrocyte death.BioMed research international · 2015Review
- Identification of the Ca²⁺ entry pathway involved in deoxygenation-induced phosphatidylserine exposure in red blood cells from patients with sickle cell disease.Pflugers Archiv : European journal of physiology · 2013Article
- The conductance of red blood cells from sickle cell patients: ion selectivity and inhibitors.The Journal of physiology · 2012Article
- The Na+/H+ exchanger controls deoxycholic acid-induced apoptosis by a H+-activated, Na+-dependent ionic shift in esophageal cells.PloS one · 2011Article
- Increased cation conductance in human erythrocytes artificially aged by glycation.The Journal of membrane biology · 2010Article
- Schistosoma mansoni soluble egg antigens trigger erythrocyte cell death.Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology · 2010Article
- Accelerated suicidal erythrocyte death in Klotho-deficient mice.Pflugers Archiv : European journal of physiology · 2009Article
- Adenosine protects against suicidal erythrocyte death.Pflugers Archiv : European journal of physiology · 2007Article
- Inhibition of eryptosis and intraerythrocytic growth of Plasmodium falciparum by flufenamic acid.Naunyn-Schmiedeberg's archives of pharmacology · 2007Article
- Induction of eryptosis by cyclosporine.Naunyn-Schmiedeberg's archives of pharmacology · 2006Article
- Potential roles of electrogenic ion transport and plasma membrane depolarization in apoptosis.The Journal of membrane biology · 2006Review
- Inhibition of erythrocyte "apoptosis" by catecholamines.Naunyn-Schmiedeberg's archives of pharmacology · 2005Article
- Ion channels in cell proliferation and apoptotic cell death.The Journal of membrane biology · 2005Review
- Channel-induced apoptosis of infected host cells-the case of malaria.Pflugers Archiv : European journal of physiology · 2004Review
- Organic osmolyte permeabilities of the malaria-induced anion conductances in human erythrocytes.The Journal of general physiology · 2004Article
- Cation channels, cell volume and the death of an erythrocyte.Pflugers Archiv : European journal of physiology · 2003Review
- Reduced DIDS-sensitive chloride conductance in Ae1-/- mouse erythrocytes.Blood cells, molecules & diseasesArticle
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Even though lacking mitochondria and nuclei erythrocytes do undergo apoptotic cell death which is characterized by breakdown of phosphatidylserine asymmetry (leading to annexin binding), membrane blebbing and cell shrinkage. Previously, we have shown that erythrocyte apoptosis is triggered by osmotic shrinkage at least in part through activation of cell volume-sensitive cation channels and subsequent Ca2+ entry. The channels could not only be activated by cell shrinkage but as well by replacement of Cl- with gluconate. Both, channel activity and annexin binding were sensitive to high concentrations of amiloride (1 mM). The present study has been performed to search for more effective blockers. To this end channel activity has been evaluated utilizing whole-cell patch-clamp and annexin binding determined by FACS analysis as an indicator of erythrocyte apoptosis. It is shown that either, increase of osmolarity or replacement of Cl- by gluconate triggers the activation of the cation channel which is inhibited by amiloride at 1 mM but not at 100 microM. Surprisingly, the cation channel was significantly more sensitive to the amiloride analogue ethylisopropylamiloride (EIPA, IC(50)=0.6+/-0.1 microM, n=5). Exposure of the cells to osmotic shock by addition of sucrose (850 mOsm) led to stimulation of annexin binding which was inhibited similarly by EIPA (IC(50)=0.2+/-0.2 microM, n=4). Moreover, annexin binding was inhibited by higher concentrations of HOE 642 (IC(50)=10+/-5 microM, n=5) and HOE 694 (IC(50)=12+/-6 microM, n=4). It is concluded that osmotic shock stimulates a cation channel which participates in the triggering of erythrocyte apoptosis. EIPA is an effective inhibitor of this cation channel and of channel mediated triggering of erythrocyte apoptosis.
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