ArticleThe Biochemical journal2003
Deep-apical tubules: dynamic lipid-raft microdomains in the brush-border region of enterocytes.
Article in The Biochemical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 60 citations in OpenAlex.
- Plasma membrane invaginations and their diverse cellular functions.BMC biology · 2026Review
- Intestinal Neutral Ceramidase Deficiency Triggers Regulatory T Cell Response via Gd3 to Protect the Host from Intestinal Inflammation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Receptor-mediated transcytosis for brain delivery of therapeutics: receptor classes and criteria.Frontiers in drug delivery · 2024Review
- Membrane Lipids in Epithelial Polarity: Sorting out the PIPs.Frontiers in cell and developmental biology · 2022Review
- Sterols lower energetic barriers of membrane bending and fission necessary for efficient clathrin-mediated endocytosis.Cell reports · 2021Article
- Sphingolipids and plasma membrane hydrolases in human primary bronchial cells during differentiation and their altered patterns in cystic fibrosis.Glycoconjugate journal · 2020Article
- Antilipidemic Drug Therapy Today and in the Future.Handbook of experimental pharmacology · 2016Review
- Probing endocytosis from the enterocyte brush border using fluorescent lipophilic dyes: lipid sorting at the apical cell surface.Histochemistry and cell biology · 2015Article
- The Annexin A2/p11 complex is required for efficient invasion of Salmonella Typhimurium in epithelial cells.Cellular microbiology · 2014Article
- Staphylococcus aureus enterotoxins A- and B: binding to the enterocyte brush border and uptake by perturbation of the apical endocytic membrane traffic.Histochemistry and cell biology · 2013Article
- Generation of stable lipid raft microdomains in the enterocyte brush border by selective endocytic removal of non-raft membrane.PloS one · 2013Article
- Glycosphingolipids are essential for intestinal endocytic function.The Journal of biological chemistry · 2012Article
- Coordinated regulation of caveolin-1 and Rab11a in apical recycling compartments of polarized epithelial cells.Experimental cell research · 2012Article
- Steroidogenic acute regulatory-related lipid transfer domain protein 5 localization and regulation in renal tubules.American journal of physiology. Renal physiology · 2009Article
- Origins of intestinal ABCA1-mediated HDL-cholesterol.Journal of lipid research · 2008Article
- Enterotoxigenic Escherichia coli vesicles target toxin delivery into mammalian cells.The EMBO journal · 2004Article
- Article
- Commensal bacterial internalization by epithelial cells: An alternative portal for gut leakiness.Tissue barriersArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The brush border of small intestinal enterocytes is highly enriched in cholesterol- and glycosphingolipid-containing membrane microdomains, commonly termed as lipid 'rafts'. Functionally, transcytosis of IgA and exocytosis of newly made brush-border proteins in enterocytes occur through apical lipid raft-containing compartments, but little is otherwise known about these raft microdomains. We therefore studied in closer detail apical lipid-raft compartments in enterocytes by immunogold electron microscopy and biochemical analyses. Novel membrane structures, deep-apical tubules, were visualized by the non-permeable surface marker Ruthenium Red in the brush-border region of the cells. The surface-connected tubules were labelled by antibodies to caveolin-1 and the glycolipid asialo G(M1), and they were sensitive to cholesterol depletion by methyl-beta-cyclodextrin, indicating the presence of raft microdomains. Deep-apical tubules were positioned close to the actin rootlets of adjacent microvilli in the terminal web region, which had a diameter of 50-100 nm, and penetrated up to 1 microm into the cytoplasm. Markers for transcytosis, IgA and the polymeric immunoglobulin receptor, as well as the resident brush-border enzyme aminopeptidase N, were present in these deep-apical tubules. We propose that deep-apical tubules are a specialized lipid-raft microdomain in the brush-border region functioning as a hub in membrane trafficking at the brush border. In addition, the sensitivity to cholesterol depletion suggests that deep-apical tubules function as a cell-surface membrane reservoir for cholesterol and for rapid adaptive changes in the size of microvilli at the brush border.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.