ArticleMolecular biology of the cell2003
Morphology and dynamics of clathrin/GGA1-coated carriers budding from the trans-Golgi network.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
54 citing papers in PubMed, 124 citations in OpenAlex.
- From Pits to Plaques: Electron Microscopy Reveals Clathrin Structural Plasticity in Trafficking, Sorting and Adhesion.Traffic (Copenhagen, Denmark) · 2026Review
- Protein sorting from endosomes to the TGN.Frontiers in cell and developmental biology · 2023Review
- Visualizing intra-Golgi localization and transport by side-averaging Golgi ministacks.The Journal of cell biology · 2022Article
- Clathrin packets move in slow axonal transport and deliver functional payloads to synapses.Neuron · 2021Article
- Inactivation of the three GGA genes in HeLa cells partially compromises lysosomal enzyme sorting.FEBS open bio · 2021Article
- Role of clathrin-mediated endocytosis in the use of heme and hemoglobin by the fungal pathogen Cryptococcus neoformans.Cellular microbiology · 2019Article
- A versatile nanobody-based toolkit to analyze retrograde transport from the cell surface.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Segregation in the Golgi complex precedes export of endolysosomal proteins in distinct transport carriers.The Journal of cell biology · 2017Article
- A novel physiological role for ARF1 in the formation of bidirectional tubules from the Golgi.Molecular biology of the cell · 2017Article
- Downregulation of a GPCR by β-Arrestin2-Mediated Switch from an Endosomal to a TGN Recycling Pathway.Cell reports · 2016Article
- siRNA Screen Identifies Trafficking Host Factors that Modulate Alphavirus Infection.PLoS pathogens · 2016Article
- Distinct Biochemical Pools of Golgi Phosphoprotein 3 in the Human Breast Cancer Cell Lines MCF7 and MDA-MB-231.PloS one · 2016Article
- The clathrin-binding and J-domains of GAK support the uncoating and chaperoning of clathrin by Hsc70 in the brain.Journal of cell science · 2015Article
- Review
- Application of Gaussia luciferase in bicistronic and non-conventional secretion reporter constructs.BMC biochemistry · 2014Article
- Gβ1γ2 activates phospholipase AFrontiers in cell and developmental biology · 2014Article
- Arf6 regulation of Gyrating-clathrin.Traffic (Copenhagen, Denmark) · 2013Article
- Article
- Regulation of the Golgi complex by phospholipid remodeling enzymes.Biochimica et biophysica acta · 2012Review
- Phosphoinositide-mediated clathrin adaptor progression at the trans-Golgi network.Nature cell biology · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Sorting of transmembrane proteins and their ligands at various compartments of the endocytic and secretory pathways is mediated by selective incorporation into clathrin-coated intermediates. Previous morphological and biochemical studies have shown that these clathrin-coated intermediates consist of spherical vesicles with a diameter of 60-100 nm. Herein, we report the use of fluorescent imaging of live cells to demonstrate the existence of a different type of transport intermediate containing associated clathrin coats. Clathrin and the adaptors GGA1 and adaptor protein-1, labeled with different spectral variants of the green fluorescent protein, are shown to colocalize to the trans-Golgi network and to a population of vesicles and tubules budding from it. These intermediates are highly pleiomorphic and move toward the peripheral cytoplasm for distances of up to 10 microm with average speeds of approximately 1 microm/s. The labeled clathrin and GGA1 cycle on and off membranes with half-times of 10-20 s, independently of vesicle budding. Our observations indicate the existence of a novel type of trans-Golgi network-derived carriers containing associated clathrin, GGA1 and adaptor protein-1 that are larger than conventional clathrin-coated vesicles, and that undergo long-range translocation in the cytoplasm before losing their coats.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.