ArticleMolecular biology of the cell2003
The microtubule plus-end proteins EB1 and dynactin have differential effects on microtubule polymerization.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
82 citing papers in PubMed, 172 citations in OpenAlex.
- Millimeter-Wave Directed Energy-Mediated Neural Cell Injury: Insights into Protein Degradation and Cell Injury Mechanisms.Research square · 2025Article
- Tubulin Complexity in Cancer and Metastasis.Advances in experimental medicine and biology · 2024Review
- End-binding protein 1 promotes specific motor-cargo association in the cell body prior to axonal delivery of dense core vesicles.Current biology : CB · 2023Article
- End Binding protein 1 promotes specific motor-cargo association in the cell body prior to axonal delivery of Dense Core Vesicles.bioRxiv : the preprint server for biology · 2023Article
- Targeting the Microtubule EB1-CLASP2 Complex Modulates NaCirculation research · 2021Article
- The Role of Microtubules in Pancreatic Cancer: Therapeutic Progress.Frontiers in oncology · 2021Review
- Microtubule self-organisation during seed germination in Arabidopsis.BMC biology · 2020Article
- New phenotype of DCTN1-related spectrum: early-onset dHMN plus congenital foot deformity.Annals of clinical and translational neurology · 2020Article
- NMR resonance assignment and structure prediction of the C-terminal domain of the microtubule end-binding protein 3.PloS one · 2020Article
- Combinatorial regulation of the balance between dynein microtubule end accumulation and initiation of directed motility.The EMBO journal · 2017Article
- Article
- DCTN1-related neurodegeneration: Perry syndrome and beyond.Parkinsonism & related disorders · 2017Review
- Polarity of varicosity initiation in central neuron mechanosensation.The Journal of cell biology · 2017Article
- Microtubule plus-end tracking of end-binding protein 1 (EB1) is regulated by CDK5 regulatory subunit-associated protein 2.The Journal of biological chemistry · 2017Article
- Structural biology of supramolecular assemblies by magic-angle spinning NMR spectroscopy.Quarterly reviews of biophysics · 2017Review
- Dynactin has two antagonistic regulatory domains and exerts opposing effects on dynein motility.PloS one · 2017Article
- Phosphorylation of EB2 by Aurora B and CDK1 ensures mitotic progression and genome stability.Nature communications · 2016Article
- GAR22β regulates cell migration, sperm motility, and axoneme structure.Molecular biology of the cell · 2016Article
- Compartmentalized Toxoplasma EB1 bundles spindle microtubules to secure accurate chromosome segregation.Molecular biology of the cell · 2015Article
- The dynamics of plus end polarization and microtubule assembly during Xenopus cortical rotation.Developmental biology · 2015Article
22 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Several microtubule-binding proteins including EB1, dynactin, APC, and CLIP-170 localize to the plus-ends of growing microtubules. Although these proteins can bind to microtubules independently, evidence for interactions among them has led to the hypothesis of a plus-end complex. Here we clarify the interaction between EB1 and dynactin and show that EB1 binds directly to the N-terminus of the p150(Glued) subunit. One function of a plus-end complex may be to regulate microtubule dynamics. Overexpression of either EB1 or p150(Glued) in cultured cells bundles microtubules, suggesting that each may enhance microtubule stability. The morphology of these bundles, however, differs dramatically, indicating that EB1 and dynactin may act in different ways. Disruption of the dynactin complex augments the bundling effect of EB1, suggesting that dynactin may regulate the effect of EB1 on microtubules. In vitro assays were performed to elucidate the effects of EB1 and p150(Glued) on microtubule polymerization, and they show that p150(Glued) has a potent microtubule nucleation effect, whereas EB1 has a potent elongation effect. Overall microtubule dynamics may result from a balance between the individual effects of plus-end proteins. Differences in the expression and regulation of plus-end proteins in different cell types may underlie previously noted differences in microtubule dynamics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.