ArticleMolecular biology of the cell2003
IGF-I-induced differentiation of L6 myogenic cells requires the activity of cAMP-phosphodiesterase.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 28 citations in OpenAlex.
- Padua Days on Muscle and Mobility Medicine, March 25-29, 2025, Hotel Petrarca, Euganean Thermae, Italy: Program and Abstracts.European journal of translational myology · 2025Article
- Systems for Muscle Cell Differentiation: From Bioengineering to Future Food.Micromachines · 2021Review
- Transcriptomic Profile of Primary Culture of Skeletal Muscle Cells Isolated from Semitendinosus Muscle of Beef and Dairy Bulls.International journal of molecular sciences · 2020Article
- Neurohypophyseal Hormones: Novel Actors of Striated Muscle Development and Homeostasis.European journal of translational myology · 2014Review
- Muscle acellular scaffold as a biomaterial: effects on C2C12 cell differentiation and interaction with the murine host environment.Frontiers in physiology · 2014Article
- Skeletal muscle tissue engineering: best bet or black beast?Frontiers in physiology · 2014Article
- Restoration versus reconstruction: cellular mechanisms of skin, nerve and muscle regeneration compared.Regenerative medicine research · 2013Review
- Opposite roles of myocardin and atrogin-1 in L6 myoblast differentiation.Journal of cellular physiology · 2013Article
- The myogenic kinome: protein kinases critical to mammalian skeletal myogenesis.Skeletal muscle · 2011Article
- Compartmentalization of beta-adrenergic signals in cardiomyocytes.Circulation research · 2011Review
- Effect of DLK1 and RTL1 but not MEG3 or MEG8 on muscle gene expression in Callipyge lambs.PloS one · 2009Article
- Article
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Inhibition of type 4 cAMP-specific phosphodiesterase (PDE4) activity in L6-C5 and L6-E9 abolished myogenic differentiation induced by low-serum medium and IGF-I. L6-C5 cells cultured in low-serum medium displayed a PDE4 activity higher than cells cultured in serum-free medium, a condition not sufficient to induce differentiation. In the presence of serum, PDE4D3, the major isoform natively expressed in L6-C5 cells, translocated to a Triton-insoluble fraction, which increased the PDE specific activity of the fraction, and exhibited a Mr shift typical of phosphorylation of this isoform. Furthermore, serum promoted the localization of PDE4D3 to a vesicular subcellular compartment. In L6-C5 cells, IGF-I is a stronger inducer of myogenic differentiation in the presence than in absence of serum. Its ability to trigger differentiation in the absence of serum was restored by overexpressing wild-type PDE4D3, but not a phosphorylation-insensitive mutant. This finding was confirmed in single cells overexpressing a GFP-PDE4D3 fusion protein by assessing nuclear accumulation of myogenin in both L6-C5 and L6-E9. Overexpression of other PDE isoforms was less efficient, confirming that PDE4D3 is the physiologically relevant phosphodiesterase isoform in the control of myogenesis. These results show that downregulation of cAMP signaling through cAMP-phosphodiesterase stimulation is a prerequisite for induction of myogenesis.
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