ArticleMolecular biology of the cell2003
The synaptojanin-like protein Inp53/Sjl3 functions with clathrin in a yeast TGN-to-endosome pathway distinct from the GGA protein-dependent pathway.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 55 citations in OpenAlex.
- Subunits of adaptor protein complex-1 make distinct contributions to the virulence ofInfection and immunity · 2026Article
- Rethinking the Yeast Endomembrane System.Sub-cellular biochemistry · 2026Review
- P4-ATPase endosomal recycling relies on multiple retromer-dependent localization signals.Molecular biology of the cell · 2024Article
- Up-regulation of Osh6 boosts an anti-aging membrane trafficking pathway toward vacuoles.Microbial cell (Graz, Austria) · 2022Article
- New directions for the clathrin adaptor AP-1 in cell biology and human disease.Current opinion in cell biology · 2022Review
- Exomer complex regulates protein traffic at the TGN through differential interactions with cargos and clathrin adaptor complexes.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Quantitative high-content imaging identifies novel regulators of Neo1 trafficking at endosomes.Molecular biology of the cell · 2017Article
- Characterization of a Novel Prevacuolar Compartment in Neurospora crassa.Eukaryotic cell · 2015Article
- Calcineurin regulates the yeast synaptojanin Inp53/Sjl3 during membrane stress.Molecular biology of the cell · 2015Article
- Mutant strains of Pichia pastoris with enhanced secretion of recombinant proteins.Biotechnology letters · 2013Article
- The Sac domain-containing phosphoinositide phosphatases: structure, function, and disease.Frontiers in biology · 2013Article
- Phosphoinositides: tiny lipids with giant impact on cell regulation.Physiological reviews · 2013Review
- The 5-phosphatase OCRL mediates retrograde transport of the mannose 6-phosphate receptor by regulating a Rac1-cofilin signalling module.Human molecular genetics · 2012Article
- Phosphoinositide-mediated clathrin adaptor progression at the trans-Golgi network.Nature cell biology · 2012Article
- Membrane-trafficking sorting hubs: cooperation between PI4P and small GTPases at the trans-Golgi network.Trends in cell biology · 2011Review
- Phosphoinositide phosphatases in cell biology and disease.Progress in lipid research · 2010Review
- Yeast Golgi-localized, gamma-Ear-containing, ADP-ribosylation factor-binding proteins are but adaptor protein-1 is not required for cell-free transport of membrane proteins from the trans-Golgi network to the prevacuolar compartment.Molecular biology of the cell · 2008Article
- Yarrowia lipolytica vesicle-mediated protein transport pathways.BMC evolutionary biology · 2007Article
- Ent3p and Ent5p exhibit cargo-specific functions in trafficking proteins between the trans-Golgi network and the endosomes in yeast.Molecular biology of the cell · 2007Article
- Avl9p, a member of a novel protein superfamily, functions in the late secretory pathway.Molecular biology of the cell · 2007Article
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
Abstract
Yeast TGN resident proteins that frequently cycle between the TGN and endosomes are much more slowly transported to the prevacuolar/late endosomal compartment (PVC) than other proteins. However, TGN protein transport to the PVC is accelerated in mutants lacking function of Inp53p. Inp53p contains a SacI polyphosphoinositide phosphatase domain, a 5-phosphatase domain, and a proline-rich domain. Here we show that all three domains are required to mediate "slow delivery" of TGN proteins into the PVC. Although deletion of the proline-rich domain did not affect general membrane association, it caused localization to become less specific. The proline-rich domain was shown to bind to two proteins, including clathrin heavy chain, Chc1p. Unlike chc1 mutants, inp53 mutants do not mislocalize TGN proteins to the cell surface, consistent with the idea that Chc1p and Inp53p act at a common vesicular trafficking step but that Chc1p is used at other steps also. Like mutations in the AP-1 adaptor complex, mutations in INP53 exhibit synthetic growth and transport defects when combined with mutations in the GGA proteins. Taken together with other recent studies, our results suggest that Inp53p and AP-1/clathrin act together in a TGN-to-early endosome pathway distinct from the direct TGN-to-PVC pathway mediated by GGA/clathrin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.