ArticleMolecular biology of the cell2003
Procyclin null mutants of Trypanosoma brucei express free glycosylphosphatidylinositols on their surface.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 55 citations in OpenAlex.
- Free GPIs and Comparison of GPI Structures Among Species.International journal of molecular sciences · 2025Review
- Common and unique features of glycosylation and glycosyltransferases in African trypanosomes.The Biochemical journal · 2022Article
- Article
- Persistence of Trypanosoma brucei as early procyclic forms and social motility are dependent on glycosylphosphatidylinositol transamidase.Molecular microbiology · 2022Article
- Article
- The Glycosylphosphatidylinositol Anchor: A Linchpin for Cell Surface Versatility of Trypanosomatids.Frontiers in cell and developmental biology · 2021Review
- Flagellar cAMP signaling controls trypanosome progression through host tissues.Nature communications · 2019Article
- Global Profiling and Inhibition of Protein Lipidation in Vector and Host Stages of the Sleeping Sickness ParasiteACS infectious diseases · 2016Article
- Identification of a glycosylphosphatidylinositol anchor-modifying β1-3 galactosyltransferase in Trypanosoma brucei.Glycobiology · 2015Article
- Social motility of African trypanosomes is a property of a distinct life-cycle stage that occurs early in tsetse fly transmission.PLoS pathogens · 2014Article
- Inhibition of nucleotide sugar transport in Trypanosoma brucei alters surface glycosylation.The Journal of biological chemistry · 2013Article
- A cell-surface phylome for African trypanosomes.PLoS neglected tropical diseases · 2013Article
- The lipid-linked oligosaccharide donor specificities of Trypanosoma brucei oligosaccharyltransferases.Glycobiology · 2012Article
- The trypanosomatid-specific N terminus of RPA2 is required for RNA polymerase I assembly, localization, and function.Eukaryotic cell · 2012Article
- Modeling of the N-glycosylated transferrin receptor suggests how transferrin binding can occur within the surface coat of Trypanosoma brucei.PLoS pathogens · 2012Article
- Transcript expression analysis of putative Trypanosoma brucei GPI-anchored surface proteins during development in the tsetse and mammalian hosts.PLoS neglected tropical diseases · 2012Article
- Article
- A structural domain mediates attachment of ethanolamine phosphoglycerol to eukaryotic elongation factor 1A in Trypanosoma brucei.PloS one · 2010Article
- PSSA-2, a membrane-spanning phosphoprotein of Trypanosoma brucei, is required for efficient maturation of infection.PloS one · 2009Article
- Fate of glycosylphosphatidylinositol (GPI)-less procyclin and characterization of sialylated non-GPI-anchored surface coat molecules of procyclic-form Trypanosoma brucei.Eukaryotic cell · 2009Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Procyclins are abundant, glycosylphosphatidylinositol (GPI)-anchored proteins on the surface of procyclic (insect) form trypanosomes. To investigate whether trypanosomes are able to survive without a procyclin coat, all four procyclin genes were deleted sequentially. Bloodstream forms of the null mutant exhibited no detectable phenotype and were able to differentiate to procyclic forms. Initially, differentiated null mutant cells were barely able to grow, but after an adaptation period of 2 mo in culture they proliferated at the same rate as wild-type trypanosomes. Analysis of these culture-adapted null mutants revealed that they were covered by free GPIs. These were closely related to the mature procyclin anchor in structure and were expressed on the surface in numbers comparable with that of procyclin in wild-type cells. However, free GPIs were smaller than the procyclin anchor, indicative of a lower number of poly-N-acetyllactosamine repeats, and a proportion contained diacylphosphatidic acid. Free GPIs are also expressed by wild-type cells, although to a lesser extent. These have been overlooked in the past because they partition in a solvent fraction (chloroform/water/methanol) that is normally discarded when GPI-anchored proteins are purified.
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Registered trials
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