ArticleCurrent genetics2003
Pitfalls of the synthetic lethality screen in Saccharomyces cerevisiae: an improved design.
Article in Current genetics, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 22 citations in OpenAlex.
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- Factors enforcing the species boundary between the human pathogens Cryptococcus neoformans and Cryptococcus deneoformans.PLoS genetics · 2021Article
- Defective RNA polymerase III is negatively regulated by the SUMO-Ubiquitin-Cdc48 pathway.eLife · 2018Article
- Highly parallel genome variant engineering with CRISPR-Cas9.Nature genetics · 2018Article
- The ribosome assembly gene network is controlled by the feedback regulation of transcription elongation.Nucleic acids research · 2017Article
- Identification of Genes inG3 (Bethesda, Md.) · 2017Article
- A genetic screen for high copy number suppressors of the synthetic lethality between elg1Δ and srs2Δ in yeast.G3 (Bethesda, Md.) · 2013Article
- Mutational analysis of flucytosine resistance in Candida glabrata.Antimicrobial agents and chemotherapy · 2010Article
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- Molecular mechanism of flucytosine resistance in Candida lusitaniae: contribution of the FCY2, FCY1, and FUR1 genes to 5-fluorouracil and fluconazole cross-resistance.Antimicrobial agents and chemotherapy · 2007Article
- Deletion of RNQ1 gene reveals novel functional relationship between divergently transcribed Bik1p/CLIP-170 and Sfi1p in spindle pole body separation.Current genetics · 2006Article
- Revisiting purine-histidine cross-pathway regulation in Saccharomyces cerevisiae: a central role for a small molecule.Genetics · 2005Article
- ELG1, a yeast gene required for genome stability, forms a complex related to replication factor C.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The colony color assay in yeast enables the visual identification of plasmid-loss events. In combination with a plasmid-dependence assay, it is commonly used to identify synthetic interactions between functionally related genes. Frequently, the plasmid carries the ADE3 gene and mutants are recognized as red colonies that fail to produce sectors. In these assays, a high percentage of false-positives is obtained, most of which result from synthetic lethality with the ade3 mutation. Here, we study the nature of these mutants. We report that mutations in the HIP1 and SHM1 genes exhibit synthetic lethality with ade3 deletions. A similar interaction is found between the fur1 and ura3 mutations. Lethality in the absence of the mitochondrial Shm1 and the cytoplasmic Ade3 enzymes indicates that, under certain circumstances, these cellular compartments cooperate in carrying out essential metabolic processes. In addition, we report the identification of a truncated ADE3 allele with a unique coloration phenotype and show that it can be used to improve synthetic lethal screens.
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