ArticleCurrent genetics2003
Analysis of altered G-protein subunit accumulation in Cryphonectria parasitica reveals a third Galpha homologue.
Article in Current genetics, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- Review
- The Destructive Fungal PathogenPathogens (Basel, Switzerland) · 2020Review
- Mutagenesis of the catalytic and cleavage site residues of the hypovirus papain-like proteases p29 and p48 reveals alternative processing and contributions to optimal viral RNA accumulation.Journal of virology · 2014Article
- Cloning and functional analysis of the Gβ gene Mgb1 and the Gγ gene Mgg1 in Monascus ruber.Journal of microbiology (Seoul, Korea) · 2014Article
- Δ(1)-pyrroline-5-carboxylate/glutamate biogenesis is required for fungal virulence and sporulation.PloS one · 2013Article
- Interaction of the heterotrimeric G protein alpha subunit SSG-1 of Sporothrix schenckii with proteins related to stress response and fungal pathogenicity using a yeast two-hybrid assay.BMC microbiology · 2010Article
- Phosphorylation of phosducin-like protein BDM-1 by protein kinase 2 (CK2) is required for virulence and G beta subunit stability in the fungal plant pathogen Cryphonectria parasitica.Molecular microbiology · 2010Article
- Controlled gene expression in the plant pathogen Cryphonectria parasitica by use of a copper-responsive element.Applied and environmental microbiology · 2009Article
- G(alpha) and Gbeta proteins regulate the cyclic AMP pathway that is required for development and pathogenicity of the phytopathogen Mycosphaerella graminicola.Eukaryotic cell · 2009Article
- Cytosolic phospholipase A2: a member of the signalling pathway of a new G protein alpha subunit in Sporothrix schenckii.BMC microbiology · 2009Article
- Article
- The phosducin-like protein PhnA is required for Gbetagamma-mediated signaling for vegetative growth, developmental control, and toxin biosynthesis in Aspergillus nidulans.Eukaryotic cell · 2006Article
- Article
- Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Heterotrimeric G-proteins mediate many responses of eukaryotic cells to external stimuli and have been shown to be important for fungal pathogenicity. In this study, we explored the accumulation of G-protein subunits of the chestnut blight fungus, Cryphonectria parasitica, in mutant strains deleted for one or more putative partner subunits. Using a series of extraction buffers and immunoblot end-point dilution analysis, we established a convenient method to assess the relative abundance of these membrane-associated proteins. Disruption of either cpg-1, which encodes the Galpha subunit CPG-1, or cpgb-1, the Gbeta subunit CPGB-1, consistently reduced the level of its presumptive partner protein. This was not observed in the case of a second Galpha subunit, CPG-2, suggesting that CPG-1 and CPGB-1 regulate each other's stability. Further, analysis of transcript levels indicated that the Galpha and Gbeta protein turnover rates were increased in the mutant strains. Additionally, a previously unidentified protein that was cross-reactive with anti-CPG-1 antiserum was found to be enhanced in liquid culture. We describe the sequence of a new Galpha subunit, CPG-3, that is most similar to three other filamentous fungal Galpha proteins that form a phylogenetically distinct grouping.
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