ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2003
Imaging calcium entry sites and ribbon structures in two presynaptic cells.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 103 papers.
What it found
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Who cites it
103 citing papers in PubMed.
- Article
- Axonal neurotransmitter release in the regulation of myelination.Bioscience reports · 2024Review
- The Interplay between Neurotransmitters and Calcium Dynamics in Retinal Synapses during Development, Health, and Disease.International journal of molecular sciences · 2024Review
- Role of Ribeye PXDLS/T-binding cleft in normal synaptic ribbon function.bioRxiv : the preprint server for biology · 2023Article
- Functional maturation of the rod bipolar to AII-amacrine cell ribbon synapse in the mouse retina.Cell reports · 2023Article
- Article
- Roles and Sources of Calcium in Synaptic Exocytosis.Advances in neurobiology · 2023Article
- The Architecture of the Presynaptic Release Site.Advances in neurobiology · 2023Article
- Article
- Transmission at rod and cone ribbon synapses in the retina.Pflugers Archiv : European journal of physiology · 2021Review
- Development and maintenance of vision's first synapse.Developmental biology · 2021Review
- The Calcium Signaling Mechanisms in Arterial Smooth Muscle and Endothelial Cells.Comprehensive Physiology · 2021Article
- Rapid CaProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Phase-Locking Requires Efficient CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Direct Observation of Vesicle Transport on the Synaptic Ribbon Provides Evidence That Vesicles Are Mobilized and Prepared Rapidly for Release.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- An amplitude code transmits information at a visual synapse.Nature neuroscience · 2019Article
- Dynamic L-type CaBiochimica et biophysica acta. Molecular cell research · 2018Article
- The high-affinity calcium sensor synaptotagmin-7 serves multiple roles in regulated exocytosis.The Journal of general physiology · 2018Review
- CaFrontiers in cellular neuroscience · 2018Article
- Two Pools of Vesicles Associated with Synaptic Ribbons Are Molecularly Prepared for Release.Biophysical journal · 2017Article
43 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
We investigated the location of calcium entry sites and synaptic ribbons in the type-Mb goldfish bipolar neuron and the bullfrog saccular hair cell. Cells were loaded with a fast calcium indicator (Fluo-3 or Fluo-5F) and an excess of a high-affinity but slow Ca buffer (EGTA). The cell surface was imaged by evanescent field microscopy. Small fluorescent "hot spots" representing calcium entry sites appeared abruptly when a voltage step opened Ca channels and disappeared or dimmed abruptly when Ca channels closed. In bipolar cells, the fluorescence of hot spots tracked the calcium influx. Hair cells showed similar Ca hot spots. Synaptic ribbons or dense bodies were labeled by immunofluorescence with an antibody that recognizes the ribbon protein ribeye. The antibody labeled punctate structures beneath the plasma membrane. In both bipolar neurons and hair cells, the number of Ca entry sites was similar or identical to that of ribbons or dense bodies, consistent with the idea that calcium-channel clusters reside near ribbons, and that both mark active zones. In bipolar cells, the number of Ca entry sites and ribeye-positive fluorescent spots is also strikingly similar to that of exocytic active zones but significantly less than the number of total exocytic sites including solitary fusion events outside active zones. We suggest that in bipolar terminals, active zones, Ca entry sites, and synaptic ribbons all colocalize, but also that a significant number of vesicles can fuse outside active zones and, hence, independently of synaptic ribbons.
Indexed as
Identifiers
12684438PMC6742070What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.