ArticleEukaryotic cell2003
Identification of essential genes in the human fungal pathogen Aspergillus fumigatus by transposon mutagenesis.
Article in Eukaryotic cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Position Effect Variegation of Penicillium rubens Genome and Application in Heterologous Production of the Plant-Derived Anthraquinone Physcion.Microbial biotechnology · 2026Article
- A Transferable Genomic Language Model Framework for Fungal Gene Essentiality Prediction.bioRxiv : the preprint server for biology · 2026Article
- Development of a telomere vector-based approach to overcome limitations caused by lethal phenotypes in the study of essential genes in Magnaporthe oryzae.Molecular plant pathology · 2024Article
- Unlocking the magic in mycelium: Using synthetic biology to optimize filamentous fungi for biomanufacturing and sustainability.Materials today. Bio · 2023Article
- Transposon insertion mutation of Antarctic psychrotrophic fungus for red pigment production adaptive to normal temperature.Journal of industrial microbiology & biotechnology · 2022Article
- Advances in fungal chemical genomics for the discovery of new antifungal agents.Annals of the New York Academy of Sciences · 2021Review
- Genetic Manipulation and Transformation Methods forMycobiology · 2021Review
- An Overview of Genomics, Phylogenomics and Proteomics Approaches in Ascomycota.Life (Basel, Switzerland) · 2020Review
- Advances in Molecular Tools and In Vivo Models for the Study of Human Fungal Pathogenesis.Microorganisms · 2020Review
- Proteomic and Metabolomic Characteristics of Extremophilic Fungi Under Simulated Mars Conditions.Frontiers in microbiology · 2019Article
- Inducible Cell Fusion Permits Use of Competitive Fitness Profiling in the Human Pathogenic Fungus Aspergillus fumigatus.Antimicrobial agents and chemotherapy · 2019Article
- Identification of Antifungal Targets Based on Computer Modeling.Journal of fungi (Basel, Switzerland) · 2018Review
- The Distribution of Miniature Impala Elements and SIX Genes in the Fusarium Genus is Suggestive of Horizontal Gene Transfer.Journal of molecular evolution · 2017Article
- Isolation of conditional mutations in genes essential for viability of Cryptococcus neoformans.Current genetics · 2017Article
- Development of the CRISPR/Cas9 System for Targeted Gene Disruption in Aspergillus fumigatus.Eukaryotic cell · 2015Article
- Characteristics of Plant Essential Genes Allow for within- and between-Species Prediction of Lethal Mutant Phenotypes.The Plant cell · 2015Article
- Article
- Exploitation of genomics in fungicide research: current status and future perspectives.Molecular plant pathology · 2013Review
- Suitability of Vader for transposon-mediated mutagenesis in Aspergillus niger.Applied and environmental microbiology · 2011Article
- The transposon impala is activated by low temperatures: use of a controlled transposition system to identify genes critical for viability of Aspergillus fumigatus.Eukaryotic cell · 2010Article
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Authors and funding
4 authors.
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Abstract
The opportunistic pathogen Aspergillus fumigatus is the most frequent cause of deadly airborne fungal infections in developed countries. In order to identify novel antifungal-drug targets, we investigated the genome of A. fumigatus for genes that are necessary for efficient fungal growth. An artificial A. fumigatus diploid strain with one copy of an engineered impala160 transposon from Fusarium oxysporum integrated into its genome was used to generate a library of diploid strains by random in vivo transposon mutagenesis. Among 2,386 heterozygous diploid strains screened by parasexual genetics, 1.2% had a copy of the transposable element integrated into a locus essential for A. fumigatus growth. Comparison of genomic sequences flanking impala160 in these mutants with that of the genome of A. fumigatus allowed the characterization of 20 previously uncharacterized A. fumigatus genes. Among these, homologues of genes essential for Saccharomyces cerevisiae growth have been identified, as well as genes that do not have homologues in other fungal species. These results confirm that heterologous transposition using the transposable element impala is a powerful tool for functional genomics in ascomycota, and they pave the way for defining the complete set of essential genes in A. fumigatus, the first step toward target-based development of new antifungal drugs.
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