ArticleEukaryotic cell2003
Exportin 1 mediates nuclear export of the kinetoplastid spliced leader RNA.
Article in Eukaryotic cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 59 citations in OpenAlex.
- Dissecting Trypanosoma brucei RRP44 function in the maturation of segmented ribosomal RNA using a regulated genetic complementation system.Nucleic acids research · 2023Article
- Mistargeting of aggregation prone mitochondrial proteins activates a nucleus-mediated posttranscriptional quality control pathway in trypanosomes.Nature communications · 2022Article
- Nuclear mRNA maturation and mRNA export control: from trypanosomes to opisthokonts.Parasitology · 2021Review
- Parallel monitoring of RNA abundance, localization and compactness with correlative single molecule FISH on LR White embedded samples.Nucleic acids research · 2021Article
- NMD3 regulates both mRNA and rRNA nuclear export in African trypanosomes via an XPOI-linked pathway.Nucleic acids research · 2015Article
- Trypanosome MKT1 and the RNA-binding protein ZC3H11: interactions and potential roles in post-transcriptional regulatory networks.Nucleic acids research · 2014Article
- Identification of a novel lipin homologue from the parasitic protozoan Trypanosoma brucei.BMC microbiology · 2013Article
- Trypanosoma brucei BRCA2 acts in a life cycle-specific genome stability process and dictates BRC repeat number-dependent RAD51 subnuclear dynamics.Nucleic acids research · 2013Article
- Inhibition of mRNA maturation in trypanosomes causes the formation of novel foci at the nuclear periphery containing cytoplasmic regulators of mRNA fate.Journal of cell science · 2012Article
- Article
- Cell biology of the trypanosome genome.Microbiology and molecular biology reviews : MMBR · 2010Review
- TbPRMT6 is a type I protein arginine methyltransferase that contributes to cytokinesis in Trypanosoma brucei.Eukaryotic cell · 2010Article
- The role of deadenylation in the degradation of unstable mRNAs in trypanosomes.Nucleic acids research · 2009Article
- Evidence for a degradosome-like complex in the mitochondria of Trypanosoma brucei.FEBS letters · 2009Article
- SMN-assisted assembly of snRNP-specific Sm cores in trypanosomes.Genes & development · 2009Article
- A type III protein arginine methyltransferase from the protozoan parasite Trypanosoma brucei.The Journal of biological chemistry · 2009Article
- Trypanosoma brucei spliced leader RNA maturation by the cap 1 2'-O-ribose methyltransferase and SLA1 H/ACA snoRNA pseudouridine synthase complex.Molecular and cellular biology · 2009Article
- Trypanosome spliced-leader-associated RNA (SLA1) localization and implications for spliced-leader RNA biogenesis.Eukaryotic cell · 2009Article
- Assembly of the Trypanosoma brucei 60S ribosomal subunit nuclear export complex requires trypanosome-specific proteins P34 and P37.Eukaryotic cell · 2009Article
- TbRGG2, an essential RNA editing accessory factor in two Trypanosoma brucei life cycle stages.The Journal of biological chemistry · 2008Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The kinetoplastid protozoan spliced leader (SL) RNA is the common substrate pre-mRNA utilized in all trans-splicing reactions. Here we show by fluorescence in situ hybridization that the SL RNA is present in the cytoplasm of Leishmania tarentolae and Trypanosoma brucei. Treatment with the karyopherin-specific inhibitor leptomycin B was toxic to T. brucei and eliminated the cytoplasmic SL RNA, suggesting that cytoplasmic SL RNA was dependent on the nuclear exporter exportin 1 (XPO1). Ectopic expression of xpo1 with a C506S mutation in T. brucei conferred resistance to leptomycin B. A reduction in SL RNA 3' extension removal and 5' methylation of nucleotide U(4) was observed in wild-type T. brucei treated with leptomycin B, suggesting that the cytoplasmic stage is necessary for SL RNA biogenesis. This study demonstrates spatial and mechanistic similarities between the posttranscriptional trafficking of the kinetoplastid protozoan SL RNA and the metazoan cis-spliceosomal small nuclear RNAs.
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Registered trials
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