ArticleNucleic acids research2003
Multiplexed SNP genotyping using the Qbead system: a quantum dot-encoded microsphere-based assay.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Applications of Quantum Dots in Preventive Oncology.Asian Pacific journal of cancer prevention : APJCP · 2024Review
- Drug-microbiota interactions: an emerging priority for precision medicine.Signal transduction and targeted therapy · 2023Review
- The Promise of Nanotechnology in Personalized Medicine.Journal of personalized medicine · 2022Review
- Nanomaterial-assisted microfluidics for multiplex assays.Mikrochimica acta · 2022Review
- Sub-100-nm metafluorophores with digitally tunable optical properties self-assembled from DNA.Science advances · 2017Article
- Diverse Applications of Nanomedicine.ACS nano · 2017Review
- Preschool children without 7-repeat DRD4 gene more likely to develop disorganized attachment style.McGill Science undergraduate research journal : MSURJ · 2015Article
- Nanotechnology in dentistry: prevention, diagnosis, and therapy.International journal of nanomedicine · 2015Review
- Quantum dots in diagnostics and detection: principles and paradigms.The Analyst · 2014Review
- Potential applications of quantum dots in mapping sentinel lymph node and detection of micrometastases in breast carcinoma.Journal of breast cancer · 2013Article
- Recent advances in nanoparticle-based Förster resonance energy transfer for biosensing, molecular imaging and drug release profiling.International journal of molecular sciences · 2012Review
- Submicrometre geometrically encoded fluorescent barcodes self-assembled from DNA.Nature chemistry · 2012Article
- Investigation and development of quantum dot-encoded microsphere bioconjugates for DNA detection by flow cytometry.Journal of fluorescence · 2012Article
- Procedures for controlling the size, structure and optical properties of CdS quantum dots during synthesis in aqueous solution.Journal of fluorescence · 2012Article
- Quantum dot enabled molecular sensing and diagnostics.Theranostics · 2012Article
- Rare-earth upconverting nanobarcodes for multiplexed biological detection.Small (Weinheim an der Bergstrasse, Germany) · 2011Article
- Microfluidics in macro-biomolecules analysis: macro inside in a nano world.Analytical and bioanalytical chemistry · 2010Review
- Multispectral image analysis of binary encoded microspheres for highly multiplexed suspension arrays.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2010Article
- Quantum dot-based theranostics.Nanoscale · 2010Review
- Performance of fluorescent labels in sedimentation bead arrays--a comparison study.Journal of fluorescence · 2009Article
Corrections and comments
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a new method using the Qbead system for high-throughput genotyping of single nucleotide polymorphisms (SNPs). The Qbead system employs fluorescent Qdot semiconductor nanocrystals, also known as quantum dots, to encode microspheres that subsequently can be used as a platform for multiplexed assays. By combining mixtures of quantum dots with distinct emission wavelengths and intensities, unique spectral 'barcodes' are created that enable the high levels of multiplexing required for complex genetic analyses. Here, we applied the Qbead system to SNP genotyping by encoding microspheres conjugated to allele-specific oligonucleotides. After hybridization of oligonucleotides to amplicons produced by multiplexed PCR of genomic DNA, individual microspheres are analyzed by flow cytometry and each SNP is distinguished by its unique spectral barcode. Using 10 model SNPs, we validated the Qbead system as an accurate and reliable technique for multiplexed SNP genotyping. By modifying the types of probes conjugated to microspheres, the Qbead system can easily be adapted to other assay chemistries for SNP genotyping as well as to other applications such as analysis of gene expression and protein-protein interactions. With its capability for high-throughput automation, the Qbead system has the potential to be a robust and cost-effective platform for a number of applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.