ArticleProceedings of the National Academy of Sciences of the United States of America2003
Clathrin-coated pits with long, dynamin-wrapped necks upon expression of a clathrin antisense RNA.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 73 citations in OpenAlex.
- Mesoscopic elasticity controls dynamin-driven fission of lipid tubules.Scientific reports · 2024Article
- Image segmentation and separation of spectrally similar dyes in fluorescence microscopy by dynamic mode decomposition of photobleaching kinetics.BMC bioinformatics · 2022Article
- Branched actin networks are organized for asymmetric force production during clathrin-mediated endocytosis in mammalian cells.Nature communications · 2022Article
- Energy and Dynamics of Caveolae Trafficking.Frontiers in cell and developmental biology · 2020Review
- SNX18 regulates ATG9A trafficking from recycling endosomes by recruiting Dynamin-2.EMBO reports · 2018Article
- Article
- The N-Terminal Amphipathic Helix of Endophilin Does Not Contribute to Its Molecular Curvature Generation Capacity.Journal of the American Chemical Society · 2016Article
- Invited review: Mechanisms of GTP hydrolysis and conformational transitions in the dynamin superfamily.Biopolymers · 2016Review
- Molecular mechanisms regulating formation, trafficking and processing of annular gap junctions.BMC cell biology · 2016Review
- A high-throughput platform for real-time analysis of membrane fission reactions reveals dynamin function.Nature cell biology · 2015Article
- Dynamin assembly strategies and adaptor proteins in mitochondrial fission.Current biology : CB · 2013Review
- Visualizing the effect of dynamin inhibition on annular gap vesicle formation and fission.Journal of cell science · 2013Article
- Mechanics of dynamin-mediated membrane fission.Annual review of biophysics · 2013Review
- Uptake of ricinB-quantum dot nanoparticles by a macropinocytosis-like mechanism.Journal of nanobiotechnology · 2012Article
- Getting active: protein sorting in endocytic recycling.Nature reviews. Molecular cell biology · 2012Review
- A feedback loop between dynamin and actin recruitment during clathrin-mediated endocytosis.PLoS biology · 2012Article
- Endocytosis of the anthrax toxin is mediated by clathrin, actin and unconventional adaptors.PLoS pathogens · 2010Article
- Article
- Cell entry of arginine-rich peptides is independent of endocytosis.The Journal of biological chemistry · 2009Article
- Clathrin is a key regulator of basolateral polarity.Nature · 2008Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To investigate the role of clathrin in coated vesicle formation, a cell line with inducible expression of clathrin heavy chain (CHC) antisense RNA was produced. After 18 h of CHC antisense RNA expression, the internalization of transferrin was inhibited by 90%. Although the amount of CHC was reduced by only 10%, the frequency of clathrin-coated pits at the cell surface increased by a factor of 3-5, and clathrin-coated structures also accumulated on a pleiomorphic, multivesicular, endosomal compartment. Remarkably, the coated pits were connected to the cell surface by long, tubular necks wrapped by dynamin rings, and the level of dynamin in the CHC antisense RNA-expressing cells was up-regulated 10-fold. In contrast, the amount of several other proteins associated with clathrin coat formation was unaffected. Thus, this study demonstrates that CHC antisense RNA causes accumulation of clathrin-coated pits with dynamin rings around the neck in intact cells not transfected with dynamin mutants, suggesting the existence of a previously uncharacterized functional interplay between clathrin and dynamin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.