ArticleJournal of clinical microbiology2003
DNase pretreatment of master mix reagents improves the validity of universal 16S rRNA gene PCR results.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 55 citations in OpenAlex.
- Microbial biomining from asteroidal material onboard the international space station.NPJ microgravity · 2026Article
- Validating digital polymerase chain reaction for 16S rRNA gene amplification from low biomass environmental samples.ISME communications · 2025Article
- Utility of 16S rRNA PCR in the Synovial Fluid for the Diagnosis of Prosthetic Joint Infection.Annals of laboratory medicine · 2018Article
- Expression of CD64 on neutrophils can be used to predict the severity of bloodstream infection before broad range 16S rRNA PCR.Folia microbiologica · 2015Article
- Article
- Reagent and laboratory contamination can critically impact sequence-based microbiome analyses.BMC biology · 2014Article
- Article
- Non-culture-based methods to diagnose bloodstream infection: Does it work?European journal of microbiology & immunology · 2013Article
- Use of staged molecular analysis to determine causes of unexplained central nervous system infections.Emerging infectious diseases · 2013Article
- Optimizing Taq polymerase concentration for improved signal-to-noise in the broad range detection of low abundance bacteria.PloS one · 2009Article
- Diagnosis of bacteremia in whole-blood samples by use of a commercial universal 16S rRNA gene-based PCR and sequence analysis.Journal of clinical microbiology · 2009Article
- Gram stain-specific-probe-based real-time PCR for diagnosis and discrimination of bacterial neonatal sepsis.Journal of clinical microbiology · 2008Article
- Real-time quantitative broad-range PCR assay for detection of the 16S rRNA gene followed by sequencing for species identification.Journal of clinical microbiology · 2006Article
- Use of 16S ribosomal DNA PCR and denaturing gradient gel electrophoresis for analysis of the microfloras of healing and nonhealing chronic venous leg ulcers.Journal of clinical microbiology · 2004Article
- Prospective study of use of PCR amplification and sequencing of 16S ribosomal DNA from cerebrospinal fluid for diagnosis of bacterial meningitis in a clinical setting.Journal of clinical microbiology · 2004Article
- A Clinico-Microbiological Study of Prosthetic Joint Infections in an Indian Tertiary Care Hospital: Role of Universal 16S rRNA Gene Polymerase Chain Reaction and Sequencing in Diagnosis.Indian journal of orthopaedicsArticle
- A broad-range PCR technique for the diagnosis of infective endocarditis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNase I pretreatment of 16S rRNA gene PCR reagents was tested. The DNase I requirement for the elimination of false-positive results varied between 0.1 and 70 IU per master mix depending on the applied Taq polymerase. PCR sensitivity was mostly maintained when 0.1 IU of DNase I was used.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.