ArticleJournal of clinical microbiology2003
Performance characteristics of the TRUGENE HIV-1 Genotyping Kit and the Opengene DNA Sequencing System.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 72 citations in OpenAlex.
- Human immunodeficiency virus (HIV) type 1 genetic diversity in HIV positive individuals on antiretroviral therapy in a cross-sectional study conducted in Teso, Western Kenya.The Pan African medical journal · 2021Article
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- Antiretroviral drug resistance mutations among patients failing first-line treatment in Hanoi, Vietnam.Infection and drug resistance · 2019Article
- Future technologies for monitoring HIV drug resistance and cure.Current opinion in HIV and AIDS · 2017Review
- A decade of viral mutations and associated drug resistance in a population of HIV-1+ Puerto Ricans: 2002-2011.PloS one · 2017Article
- Review
- Automating HIV drug resistance genotyping with RECall, a freely accessible sequence analysis tool.Journal of clinical microbiology · 2012Article
- Genotyping external quality assurance in the World Health Organization HIV drug resistance laboratory network during 2007-2010.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2012Article
- Emergence of HIV-1 drug resistance mutations among antiretroviral-naïve HIV-1-infected patients after rapid scaling up of antiretroviral therapy in Thailand.Journal of the International AIDS Society · 2012Article
- Routine use of microbial whole genome sequencing in diagnostic and public health microbiology.PLoS pathogens · 2012Review
- Differential persistence of transmitted HIV-1 drug resistance mutation classes.The Journal of infectious diseases · 2011Article
- Transmitted drug resistance in persons with acute/early HIV-1 in San Francisco, 2002-2009.PloS one · 2010Article
- Rapid progressing allele HLA-B35 Px restricted anti-HIV-1 CD8+ T cells recognize vestigial CTL epitopes.PloS one · 2010Article
- Affordable in-house antiretroviral drug resistance assay with good performance in non-subtype B HIV-1.Journal of virological methods · 2010Article
- Dried-plasma transport using a novel matrix and collection system for human immunodeficiency virus and hepatitis C virus virologic testing.Journal of clinical microbiology · 2009Article
- Relationship of injection drug use, antiretroviral therapy resistance, and genetic diversity in the HIV-1 pol gene.Journal of acquired immune deficiency syndromes (1999) · 2009Article
- Nucleoside and nucleotide analogs select in culture for different patterns of drug resistance in human immunodeficiency virus types 1 and 2.Antimicrobial agents and chemotherapy · 2009Article
- Genotypic antiretroviral resistance testing for human immunodeficiency virus type 1 integrase inhibitors by use of the TruGene sequencing system.Journal of clinical microbiology · 2008Article
- Immunity to HIV-1 is influenced by continued natural exposure to exogenous virus.PLoS pathogens · 2008Article
- Treatment response in acute/early infection versus advanced AIDS: equivalent first and second phases of HIV RNA decline.AIDS (London, England) · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
Abstract
The TRUGENE HIV-1 Genotyping Kit and OpenGene DNA Sequencing System are designed to sequence the protease (PR)- and reverse transcriptase (RT)-coding regions of human immunodeficiency virus type 1 (HIV-1) pol. Studies were undertaken to determine the accuracy of this assay system in detecting resistance-associated mutations and to determine the effects of RNA extraction methods, anticoagulants, specimen handling, and potentially interfering substances. Samples were plasma obtained from HIV-infected subjects or seronegative plasma to which viruses derived from wild-type and mutant infectious molecular clones (IMC) of HIV-1 were added. Extraction methods tested included standard and UltraSensitive AMPLICOR HIV-1 MONITOR, QIAGEN viral RNA extraction mini kit, and QIAGEN Ultra HIV extraction kit, and NASBA manual HIV-1 quantitative NucliSens. Sequence data from test sites were compared to a "gold standard" reference sequence to determine the percent agreement. Comparisons between test and reference sequences at the nucleotide level showed 97.5 to 100% agreement. Similar results were obtained regardless of extraction method, regardless of use of EDTA or acid citrate dextrose as anticoagulant, and despite the presence of triglycerides, bilirubin, hemoglobin, antiretroviral drugs, HIV-2, hepatitis C virus (HCV), HBV, cytomegalovirus, human T-cell leukemia virus type 1 (HTLV-1), or HTLV-2. Samples with HIV-1 RNA titers of >or=1,000 copies/ml gave consistent results. The TRUGENE HIV-1 Genotyping Kit and OpenGene DNA Sequencing System consistently generate highly accurate sequence data when tested with IMC-derived HIV and patient samples.
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Registered trials
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