ArticleJournal of clinical microbiology2003
Detection of Sporothrix schenckii in clinical samples by a nested PCR assay.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 83 citations in OpenAlex.
- Sporotrichosis in Mexico.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Pooled it
- Current Insights intoJournal of fungi (Basel, Switzerland) · 2025Article
- Veterinary application of LAMP: a simple and visual detection tool for feline sporotrichosis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Detection of Sporothrix brasiliensis in Human Tissue by Direct PCR Using Species-Specific Primers and NESTED PCR in a New Epidemic Area of Sporotrichosis.Mycopathologia · 2025Article
- Extracutaneous sporotrichosis.Clinical microbiology reviews · 2025Review
- Diagnosis of Human Endemic Mycoses Caused by Thermally Dimorphic Fungi: From Classical to Molecular Methods.Journal of fungi (Basel, Switzerland) · 2024Review
- Rapid Molecular Diagnosis of Sporotrichosis Directly from Biological Samples from a Reference Center in Brazil.Journal of fungi (Basel, Switzerland) · 2024Article
- Development of loop-mediated isothermal amplification for rapid detection of sporotrichosis caused byVeterinary world · 2023Article
- Evaluation of Five Non-Culture-Based Methods for the Diagnosis of Meningeal Sporotrichosis.Journal of fungi (Basel, Switzerland) · 2023Article
- Sporotrichosis: In silico design of new molecular markers for the Sporothrix genus.Revista da Sociedade Brasileira de Medicina Tropical · 2023Article
- Trends in Molecular Diagnostics and Genotyping Tools Applied for EmergingJournal of fungi (Basel, Switzerland) · 2022Review
- Current Progress on Epidemiology, Diagnosis, and Treatment of Sporotrichosis and Their Future Trends.Journal of fungi (Basel, Switzerland) · 2022Review
- Beyond Domestic Cats: Environmental Detection ofJournal of fungi (Basel, Switzerland) · 2022Article
- Keratinocyte Response to Infection withJournal of fungi (Basel, Switzerland) · 2022Article
- Osteoarticular sporotrichosis in an immunocompetent patient.Medical mycology case reports · 2021Article
- Nested PCR for the Diagnosis of Feline Sporotrichosis From Formalin-Fixed and Paraffin-Embedded Samples Using Different DNA Extraction Protocols.Frontiers in veterinary science · 2021Article
- A One Health Approach to CombattingJournal of fungi (Basel, Switzerland) · 2020Review
- Diagnosis of Breakthrough Fungal Infections in the Clinical Mycology Laboratory: An ECMM Consensus Statement.Journal of fungi (Basel, Switzerland) · 2020Article
- The threat of emerging and re-emerging pathogenic Sporothrix species.Mycopathologia · 2020Review
- PCR-based diagnosis of Sporothrix infection using DNA from paraffin-embedded skin specimens in previously undiagnosed cases.European journal of dermatology : EJD · 2020Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cutaneous sporotrichosis is a chronic granulomatous fungal infection caused by Sporothrix schenckii with worldwide distribution. Its traditional diagnosis is time-consuming and difficult to differentiate from that of a clinical sporotrichoid lesion caused by various pathogens. In this study, a nested PCR assay for the detection of S. schenckii was evaluated by using a sequence of 18S rRNA gene as a target. For the examination of specificity and sensitivity, five clinical isolates with 1 ATCC 10213 strain of S. schenckii, 10 strains of clinical common fungi, 3 strains of Mycobacterium spp., Staphylococcus aureus, and normal human skin tissue were used. The expected fragment was amplified from six S. schenckii isolates in the first round and nested PCR but not from other microorganisms and human DNA. Their sequences were 100% identical to the S. schenckii 18S rRNA gene sequence deposited in GenBank. A detection limit of 40 fg of S. schenckii DNA extract was determined with ethidium bromide staining. Serial dilution studies demonstrated that the nested PCR could detect a DNA amount of 1 CFU of S. schenckii in tissue samples. We further investigated the nested PCR assay for the detection of S. schenckii from the tail tissues of 5 experimentally infected mice and from the clinical biopsy specimens of 12 patients with sporotrichosis confirmed by culture or histochemical staining. The nested PCR assay was positive in all 5 infected mice and in 11 of the 12 clinical specimens. The high sensitivity and specificity of this nested PCR indicate that the assay can provide rapid diagnosis with sufficient accuracy to be clinically useful for patients with sporotrichosis.
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