ArticleThe Journal of cell biology2003
Calcium signaling in a low calcium environment: how the intracellular malaria parasite solves the problem.
Article in The Journal of cell biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed.
- Calcium-binding protein TgpCaBP regulates calcium storage of the zoonotic parasiteMicrobiology spectrum · 2024Article
- Cultivation of Asexual Intraerythrocytic Stages ofPathogens (Basel, Switzerland) · 2023Review
- Changes in KInternational journal of molecular sciences · 2023Review
- The enemy within: lipid asymmetry in intracellular parasite-host interactions.Emerging topics in life sciences · 2023Review
- Novel stem cell technologies are powerful tools to understand the impact of human factors onFrontiers in cellular and infection microbiology · 2023Article
- Combining IPCurrent research in microbial sciences · 2023Article
- Overexpression ofPathogens (Basel, Switzerland) · 2021Article
- Chemical Biology Tools To Investigate Malaria Parasites.Chembiochem : a European journal of chemical biology · 2021Review
- Breakdown in membrane asymmetry regulation leads to monocyte recognition of P. falciparum-infected red blood cells.PLoS pathogens · 2021Article
- CDPKs: The critical decoders of calcium signal at various stages of malaria parasite development.Computational and structural biotechnology journal · 2021Review
- Calcium in the Backstage of Malaria Parasite Biology.Frontiers in cellular and infection microbiology · 2021Review
- The genetic CaThe Journal of biological chemistry · 2020Article
- Human plasma plasminogen internalization route in Plasmodium falciparum-infected erythrocytes.Malaria journal · 2020Article
- Human genetics and malaria resistance.Human genetics · 2020Review
- Genome-wide association studies of severe P. falciparum malaria susceptibility: progress, pitfalls and prospects.BMC medical genomics · 2019Review
- Review
- Human candidate gene polymorphisms and risk of severe malaria in children in Kilifi, Kenya: a case-control association study.The Lancet. Haematology · 2018Article
- A novel dense granule protein, GRA41, regulates timing of egress and calcium sensitivity in Toxoplasma gondii.Cellular microbiology · 2017Article
- Plasmodium falciparum GPCR-like receptor SR25 mediates extracellular KScientific reports · 2017Article
- PfCDPK1 mediated signaling in erythrocytic stages of Plasmodium falciparum.Nature communications · 2017Article
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Malaria parasites, Plasmodia, spend most of their asexual life cycle within red blood cells, where they proliferate and mature. The erythrocyte cytoplasm has very low [Ca2+] (<100 nM), which is very different from the extracellular environment encountered by most eukaryotic cells. The absence of extracellular Ca2+ is usually incompatible with normal cell functions and survival. In the present work, we have tested the possibility that Plasmodia overcome the limitation posed by the erythrocyte intracellular environment through the maintenance of a high [Ca2+] within the parasitophorous vacuole (PV), the compartment formed during invasion and within which the parasites grow and divide. Thus, Plasmodia were allowed to invade erythrocytes in the presence of Ca2+ indicator dyes. This allowed selective loading of the Ca2+ probes within the PV. The [Ca2+] within this compartment was found to be approximately 40 microM, i.e., high enough to be compatible with a normal loading of the Plasmodia intracellular Ca2+ stores, a prerequisite for the use of a Ca2+-based signaling mechanism. We also show that reduction of extracellular [Ca2+] results in a slow depletion of the [Ca2+] within the PV. A transient drop of [Ca2+] in the PV for a period as short as 2 h affects the maturation process of the parasites within the erythrocytes, with a major reduction 48 h later in the percentage of schizonts, the form that re-invades the red blood cells.
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