ArticleThe EMBO journal2003
The yeast Sgs1 helicase is differentially required for genomic and ribosomal DNA replication.
Article in The EMBO journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed, 104 citations in OpenAlex.
- Review
- Tolerance to replication stress requires Dun1p kinase and activation of the electron transport chain.Biochimica et biophysica acta. Molecular cell research · 2023Article
- Article
- Review
- Article
- The RecQ helicase Sgs1 drives ATP-dependent disruption of Rad51 filaments.Nucleic acids research · 2019Article
- Article
- RECQ-like helicases Sgs1 and BLM regulate R-loop-associated genome instability.The Journal of cell biology · 2017Article
- Chromosome Duplication in Saccharomyces cerevisiae.Genetics · 2016Review
- Reversible Top1 cleavage complexes are stabilized strand-specifically at the ribosomal replication fork barrier and contribute to ribosomal DNA stability.Nucleic acids research · 2014Article
- Resolving branched DNA intermediates with structure-specific nucleases during replication in eukaryotes.Chromosoma · 2013Review
- Three distinct modes of Mec1/ATR and Tel1/ATM activation illustrate differential checkpoint targeting during budding yeast early meiosis.Molecular and cellular biology · 2013Article
- Cohesin association to replication sites depends on rad50 and promotes fork restart.Molecular cell · 2012Article
- Association of epigenetic inactivation of the WRN gene with anticancer drug sensitivity in cervical cancer cells.Oncology reports · 2012Article
- BLM helicase facilitates RNA polymerase I-mediated ribosomal RNA transcription.Human molecular genetics · 2012Article
- dNTP pools determine fork progression and origin usage under replication stress.The EMBO journal · 2012Article
- RECQL5 cooperates with Topoisomerase II alpha in DNA decatenation and cell cycle progression.Nucleic acids research · 2012Article
- Genome instability and transcription elongation impairment in human cells depleted of THO/TREX.PLoS genetics · 2011Article
- The Swi2-Snf2-like protein Uls1 is involved in replication stress response.Nucleic acids research · 2011Article
- Differential genetic interactions between Sgs1, DNA-damage checkpoint components and DNA repair factors in the maintenance of chromosome stability.Genome integrity · 2011Article
1 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The members of the RecQ family of DNA helicases play conserved roles in the preservation of genome integrity. RecQ helicases are implicated in Bloom and Werner syndromes, which are associated with genomic instability and predisposition to cancers. The human BLM and WRN helicases are required for normal S phase progression. In contrast, Saccharomyces cerevisiae cells deleted for SGS1 grow with wild-type kinetics. To investigate the role of Sgs1p in DNA replication, we have monitored S phase progression in sgs1Delta cells. Unexpectedly, we find that these cells progress faster through S phase than their wild-type counterparts. Using bromodeoxyuridine incorporation and DNA combing, we show that replication forks are moving more rapidly in the absence of the Sgs1 helicase. However, completion of DNA replication is strongly retarded at the rDNA array of sgs1Delta cells, presumably because of their inability to prevent recombination at stalled forks, which are very abundant at this locus. These data suggest that Sgs1p is not required for processive DNA synthesis but prevents genomic instability by coordinating replication and recombination events during S phase.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.