ArticleThe EMBO journal2003
Regulation of RAG1/RAG2-mediated transposition by GTP and the C-terminal region of RAG2.
Article in The EMBO journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 67 citations in OpenAlex.
- Recombination between fragile regions associated with chromosomal rearrangements in glioblastoma can be mediated by RAGs.iScience · 2025Article
- Functional regulation of an ancestral RAG transposon ProtoRAG by a trans-acting factor YY1 in lancelet.Nature communications · 2020Article
- Calcium Signaling: From Normal B Cell Development to Tolerance Breakdown and Autoimmunity.Clinical reviews in allergy & immunology · 2017Review
- New insights into the evolutionary origins of the recombination-activating gene proteins and V(D)J recombination.The FEBS journal · 2017Review
- Article
- Real-time monitoring of RAG-catalyzed DNA cleavage unveils dynamic changes in coding end association with the coding end complex.Nucleic acids research · 2012Article
- Autoinhibition of DNA cleavage mediated by RAG1 and RAG2 is overcome by an epigenetic signal in V(D)J recombination.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- V(D)J recombination: Born to be wild.Seminars in cancer biology · 2010Review
- RAG: a recombinase diversified.Nature immunology · 2009Review
- Paleo-immunology: evidence consistent with insertion of a primordial herpes virus-like element in the origins of acquired immunity.PloS one · 2009Article
- RAG2's non-core domain contributes to the ordered regulation of V(D)J recombination.Nucleic acids research · 2008Article
- Dissecting the roles of MuB in Mu transposition: ATP regulation of DNA binding is not essential for target delivery.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Understanding how the V(D)J recombinase catalyzes transesterification: distinctions between DNA cleavage and transposition.Nucleic acids research · 2008Article
- Chromosomal reinsertion of broken RSS ends during T cell development.The Journal of experimental medicine · 2007Article
- In vivo reinsertion of excised episomes by the V(D)J recombinase: a potential threat to genomic stability.PLoS biology · 2007Article
- Target DNA structure plays a critical role in RAG transposition.PLoS biology · 2006Article
- Mobilization of RAG-generated signal ends by transposition and insertion in vivo.Molecular and cellular biology · 2006Article
- Double-strand break formation by the RAG complex at the bcl-2 major breakpoint region and at other non-B DNA structures in vitro.Molecular and cellular biology · 2005Article
- Genetic evidence that GTP is required for transposition of IS903 and Tn552 in Escherichia coli.Journal of bacteriology · 2005Article
- Guanosine triphosphate acts as a cofactor to promote assembly of initial P-element transposase-DNA synaptic complexes.Genes & development · 2005Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The RAG1 and RAG2 proteins perform critical DNA recognition and cleavage functions in V(D)J recombination, and also catalyze efficient DNA transposition in vitro. No transposition in vivo by the RAG proteins has been reported, suggesting regulation of the reaction by as yet unknown mechanisms. Here we report that RAG-mediated transposition is suppressed by physiological concentrations of the guanine nucleotide GTP, and by the full-length RAG2 protein. Both GTP and full-length RAG2 inhibit transposition by blocking the non-covalent 'capture' of target DNA, and both are capable of inhibiting RAG-mediated hybrid joint formation in vitro. We also observe that another intracellular signaling molecule, Ca(2+), stimulates RAG-mediated transposition and is capable of activating transposition even in reactions containing full-length RAG2 and GTP. RAG-mediated transposition has been proposed to contribute to the chromosomal translocations that underlie the development of lymphoid malignancies, and our findings highlight regulatory mechanisms that might prevent such occurrences, and circumstances in which these regulatory mechanisms could be overcome.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.