ArticleThe EMBO journal2003
Characterization of a novel RNA-binding region of eIF4GI critical for ribosomal scanning.
Article in The EMBO journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
46 citing papers in PubMed.
- Eukaryotic translation initiation factor 4F: functional properties and physiological role.Nucleic acids research · 2025Review
- Non-AUG HIV-1 uORF translation elicits specific T cell immune response and regulates viral transcript expression.Nature communications · 2025Article
- A translation enhancer element from black beetle virus engages yeast eIF4G1 to drive cap-independent translation initiation.Scientific reports · 2021Article
- Concerted action of two 3' cap-independent translation enhancers increases the competitive strength of translated viral genomes.Nucleic acids research · 2017Article
- microRNAs stimulate translation initiation mediated by HCV-like IRESes.Nucleic acids research · 2017Article
- Functional role of eukaryotic translation initiation factor 4 gamma 1 (EIF4G1) in NSCLC.Oncotarget · 2016Article
- Attachment of ribosomal complexes and retrograde scanning during initiation on the Halastavi árva virus IRES.Nucleic acids research · 2016Article
- Translational Control of the HIV Unspliced Genomic RNA.Viruses · 2015Review
- The role of the poly(A) binding protein in the assembly of the Cap-binding complex during translation initiation in plants.Translation (Austin, Tex.) · 2014Review
- Eukaryotic initiation factor (eIF) 4F binding to barley yellow dwarf virus (BYDV) 3'-untranslated region correlates with translation efficiency.The Journal of biological chemistry · 2014Article
- Article
- Human eukaryotic initiation factor 4G (eIF4G) protein binds to eIF3c, -d, and -e to promote mRNA recruitment to the ribosome.The Journal of biological chemistry · 2013Article
- Reinitiation and other unconventional posttermination events during eukaryotic translation.Molecular cell · 2013Article
- Cation-dependent folding of 3' cap-independent translation elements facilitates interaction of a 17-nucleotide conserved sequence with eIF4G.Nucleic acids research · 2013Article
- The current status of vertebrate cellular mRNA IRESs.Cold Spring Harbor perspectives in biology · 2013Review
- miRNA repression of translation in vitro takes place during 43S ribosomal scanning.Nucleic acids research · 2013Article
- 3' cap-independent translation enhancers of plant viruses.Annual review of microbiology · 2013Review
- Eukaryotic initiation factor 4B and the poly(A)-binding protein bind eIF4G competitively.Translation (Austin, Tex.) · 2013Article
- Sequential eukaryotic translation initiation factor 5 (eIF5) binding to the charged disordered segments of eIF4G and eIF2β stabilizes the 48S preinitiation complex and promotes its shift to the initiation mode.Molecular and cellular biology · 2012Article
- Specific domains in yeast translation initiation factor eIF4G strongly bias RNA unwinding activity of the eIF4F complex toward duplexes with 5'-overhangs.The Journal of biological chemistry · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The eukaryotic translation initiation factor eIF4GI binds several proteins and acts as a scaffold to promote preinitiation complex formation on the mRNA molecule (48S). Following mRNA attachment this complex scans along the messenger in a 5' to 3' direction until it locates and recognizes the initiation start codon. By using a combination of retroviral and picornaviral proteases (HIV-2 and L respectively) in the reticulocyte lysate system, we have characterized a 40 amino acid (aa) region of eIF4GI (aa 642-681) that exhibits general RNA-binding properties. Removal of this domain by proteolytic processing followed by translational assays showed virtually no inhibition of internal ribosome entry on the encephalomyocarditis virus, but resulted in drastic impairment of ribosome scanning as demonstrated by studying poliovirus and foot-and-mouth disease virus translation. Based on these findings, we propose that this 40 aa motif of eIF4GI is critical for ribosome scanning.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.