ArticleProceedings of the National Academy of Sciences of the United States of America2003
Isolation and identification of short nucleotide sequences that affect translation initiation in Saccharomyces cerevisiae.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 29 citations in OpenAlex.
- A hidden intrinsic ability of bicistronic expression based on a novel translation reinitiation mechanism in yeast.Nucleic acids research · 2025Article
- A polycistronic system for multiplexed and precalibrated expression of multigene pathways in fungi.Nature communications · 2023Article
- Synthetic polycistronic sequences in eukaryotes.Synthetic and systems biotechnology · 2021Review
- Synthetic biology: advancing the design of diverse genetic systems.Annual review of chemical and biomolecular engineering · 2013Review
- Synthetic RNA modules for fine-tuning gene expression levels in yeast by modulating RNase III activity.Nucleic acids research · 2011Article
- A synthetic library of RNA control modules for predictable tuning of gene expression in yeast.Molecular systems biology · 2011Article
- Chemical-genetic profile analysis of five inhibitory compounds in yeast.BMC chemical biology · 2010Article
- Frameworks for programming biological function through RNA parts and devices.Chemistry & biology · 2009Review
- Chemical-genetic profile analysis in yeast suggests that a previously uncharacterized open reading frame, YBR261C, affects protein synthesis.BMC genomics · 2008Article
- Searching for IRES.RNA (New York, N.Y.) · 2006Review
- An unusual internal ribosome entry site in the herpes simplex virus thymidine kinase gene.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- A positive feedback vector for identification of nucleotide sequences that enhance translation.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Biochemical and functional analysis of a 9-nt RNA sequence that affects translation efficiency in eukaryotic cells.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In previous studies, we demonstrated the sufficiency of short nucleotide sequences to facilitate internal initiation of translation in mammalian cells. By using a selection methodology, we have now identified comparable sequences in Saccharomyces cerevisiae. For these studies, a library of constructs expressing dicistronic mRNAs with the HIS3 gene as the second cistron and 18 random nucleotides in the intercistronic region was introduced into a yeast strain in which the endogenous HIS3 gene was deleted. Untransformed cells or those containing the parent construct failed to grow on medium lacking histidine. Intercistronic sequences recovered from cells that did grow were evaluated by using various criteria. Fifty-six of the 18-nt sequences (approximately 1/400,000) functioned as synthetic internal ribosome entry sites (IRESes). The 14 most active sequences allowed growth in the presence of 0.1-0.6 mM 3-amino-1,2,4-triazole, a competitive inhibitor of the HIS3 gene product. In addition, eight sequences were identified that were not IRESes, but that enhanced HIS3 expression by an alternative mechanism that depended on the 5' end of the mRNA and appeared to involve either shunting or reinitiation. Comparisons among the 56 selected IRESes identified eight significant sequence matches containing up to 10 nucleotides. Many of the selected sequences also contained extensive complementary matches to yeast 18S rRNA, some at overlapping sites. The identification of cis sequences that facilitate translation initiation in yeast enables detailed biochemical and genetic analyses of underlying mechanisms and may have practical applications for bioengineering.
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