ArticleThe Journal of physiology2003
Properties of a constitutively active Ca2+-permeable non-selective cation channel in rabbit ear artery myocytes.
Article in The Journal of physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
- Vascular mechanotransduction.Physiological reviews · 2023Review
- Maintenance of intracellular Ca2+ basal concentration in airway smooth muscle (Review).International journal of molecular medicine · 2018Review
- Transient receptor potential channels in the vasculature.Physiological reviews · 2015Review
- Pharmacological profile of phosphatidylinositol 3-kinases and related phosphatidylinositols mediating endothelin(A) receptor-operated native TRPC channels in rabbit coronary artery myocytes.British journal of pharmacology · 2012Article
- Orai1 and Ca2+-independent phospholipase A2 are required for store-operated Icat-SOC current, Ca2+ entry, and proliferation of primary vascular smooth muscle cells.American journal of physiology. Cell physiology · 2012Article
- Basally activated nonselective cation currents regulate the resting membrane potential in human and monkey colonic smooth muscle.American journal of physiology. Gastrointestinal and liver physiology · 2011Article
- Molecular expression and functional role of canonical transient receptor potential channels in airway smooth muscle cells.Advances in experimental medicine and biology · 2011Review
- TRPC6 channels stimulated by angiotensin II are inhibited by TRPC1/C5 channel activity through a Ca2+- and PKC-dependent mechanism in native vascular myocytes.The Journal of physiology · 2010Article
- Functional role of canonical transient receptor potential 1 and canonical transient receptor potential 3 in normal and asthmatic airway smooth muscle cells.American journal of respiratory cell and molecular biology · 2010Article
- Diverse properties of store-operated TRPC channels activated by protein kinase C in vascular myocytes.The Journal of physiology · 2008Article
- ACh-induced depolarization in inner ear artery is generated by activation of a TRP-like non-selective cation conductance and inactivation of a potassium conductance.Hearing research · 2008Article
- Store-operated calcium entry in vascular smooth muscle.British journal of pharmacology · 2008Review
- Endothelin-1 activates a Ca2+-permeable cation channel with TRPC3 and TRPC7 properties in rabbit coronary artery myocytes.The Journal of physiology · 2007Article
- Angiotensin II activates two cation conductances with distinct TRPC1 and TRPC6 channel properties in rabbit mesenteric artery myocytes.The Journal of physiology · 2006Article
- TRPC3 properties of a native constitutively active Ca2+-permeable cation channel in rabbit ear artery myocytes.The Journal of physiology · 2006Article
- Signal transduction pathways and gating mechanisms of native TRP-like cation channels in vascular myocytes.The Journal of physiology · 2006Review
- Role of phospholipase D and diacylglycerol in activating constitutive TRPC-like cation channels in rabbit ear artery myocytes.The Journal of physiology · 2005Article
- Role of basal extracellular Ca2+ entry during 5-HT-induced vasoconstriction of canine pulmonary arteries.British journal of pharmacology · 2005Article
- Extracellular Mg(2+) blocks endothelin-1-induced contraction through the inhibition of non-selective cation channels in coronary smooth muscle.Pflugers Archiv : European journal of physiology · 2004Article
- Inhibitory regulation of constitutive transient receptor potential-like cation channels in rabbit ear artery myocytes.The Journal of physiology · 2004Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In smooth muscle, non-selective cation conductances contribute to agonist-evoked depolarisation and contraction, and in the present study using patch-pipette techniques we describe the properties of a constitutively active cation channel. With whole-cell recording in K+-free conditions, there was a spontaneous current with a reversal potential (Er) that was altered by replacement of external Na+ by an impermeant cation, but not when external Cl- was replaced by an impermeant anion. The tonic cation inward current could be carried by Ca2+ ions and was greatly enhanced when the external Ca2+ concentration was reduced. In outside-out patches there was spontaneous cation channel activity that could be resolved into three conductance states of about 15, 25 and 40 pS, all with the same Er as the whole-cell current. Kinetic analysis revealed that there were two open times of about 1 and 5 ms and that the currents displayed bursting kinetics with burst durations of approximately 5 ms and 25 ms. Removal of external Ca2+ ions increased the probability of channel opening (Po) sixfold, which was associated with an increase in the longer burst duration. Bath application of the diacylglycerol analogue 1-oleoyl-2-acetyl-sn-glycerol increased Po, but phorbol 12,13-dibutyrate, which stimulates protein kinase C (PKC), reduced channel activity. In contrast, the PKC inhibitor chelerythrine increased the activity of channel currents. It is concluded that in rabbit ear artery myocytes there is a constitutively active Ca2+-permeable cation channel that is regulated by external Ca2+ ions and suppressed by tonic PKC activity. It is proposed that this mechanism may contribute to the resting membrane conductance and basal Ca2+ influx in this particular arterial preparation.
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