ArticlePlant molecular biology2003
Matrix attachment regions enhance transcription of a downstream transgene and the accessibility of its promoter region to micrococcal nuclease.
Article in Plant molecular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 28 citations in OpenAlex.
- Lettuce-based production of an oral vaccine against porcine edema disease for the seed lot system.Plant biotechnology (Tokyo, Japan) · 2021Article
- Review
- Construction of a novel synthetic root-specific promoter and its characterization in transgenic tobacco plants.3 Biotech · 2017Article
- TM6, a novel nuclear matrix attachment region, enhances its flanking gene expression through influencing their chromatin structure.Molecules and cells · 2013Article
- Functional analysis of BnMAR element in transgenic tobacco plants.Molecular biology reports · 2011Article
- Shedding light on the role of AT-hook/PPC domain protein in Arabidopsis thaliana.Plant signaling & behavior · 2010Article
- Functional characterization of a tobacco matrix attachment region-mediated enhancement of transgene expression.Transgenic research · 2009Article
- Selective matrix attachment regions in T helper cell subsets support loop conformation in the Ifng gene.Genes and immunity · 2007Article
- The rb7 matrix attachment region increases the likelihood and magnitude of transgene expression in tobacco cells: a flow cytometric study.The Plant cell · 2005Article
- UV-C response of the ribonucleotide reductase large subunit involves both E2F-mediated gene transcriptional regulation and protein subcellular relocalization in tobacco cells.Nucleic acids research · 2004Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nuclear matrix attachment regions (MARs) are thought to influence the expression of flanking genes. In this study, we investigated the activation of genes by tobacco MARs that had previously been identified in the 5' region of the basic class I chitinase gene, CHNS0. In transgenic tobacco cells, a construct consisted of the 35S promoter of cauliflower mosaic virus (CaMV) fused to a beta-glucuronidase gene (uidA) with 5' MAR elements was expressed at a 10-fold higher level than a similar construct without MAR sequences. However, expression of a similar construct with 3' MARs and of a construct with a truncated (-46) 35S minimal promoter and uidA with 5' MARs was not similarly enhanced, suggesting that MARs might act by increasing the activity of downstream enhancers. Deletion analysis of the MAR sequences revealed that the function of the MARs that increased the expression of the transgene was redundant. Moreover, assays of the transient expression of transgenes suggested that MAR elements might be involved in the structure and organization of chromatin. To examine the influence of MARs on chromatin structure, we investigated the effects of micrococcal nuclease (MNase) on the DNA in the reporter gene around the MARs. Analysis of the time-course of digestion of nuclei with MNase revealed that the 35S promoter region with 5' MARs was much more sensitive to MNase than the same region without MARs, suggesting that MARs might mediate the opening of chromatin in the region of a downstream promoter, with consequent enhancement of transcription.
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