ArticleCurrent genetics2003
Functional characterization of the Frt1 sugar transporter and of fructose uptake in Kluyveromyces lactis.
Article in Current genetics, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 38 citations in OpenAlex.
- Coupling Carbon-Source Transport with Antioxidant Biosynthesis in Yeast: Product Diversity and Engineering Strategies.Biology · 2026Review
- Evaluation of Stress Tolerance and Fermentation Performance in Commercial Yeast Strains for Industrial Applications.Foods (Basel, Switzerland) · 2025Article
- Molecular Modification Enhances Xylose Uptake by the Sugar Transporter KM_SUT5 ofInternational journal of molecular sciences · 2024Article
- Engineering proton-coupled hexose uptake in Saccharomyces cerevisiae for improved ethanol yield.Biotechnology for biofuels and bioproducts · 2022Article
- D-Fructose Assimilation and Fermentation by Yeasts Belonging to Saccharomycetes: Rediscovery of Universal Phenotypes and Elucidation of Fructophilic Behaviors inMicroorganisms · 2021Article
- Construction of engineered Saccharomyces cerevisiae strain to improve that whole-cell biocatalytic production of melibiose from raffinose.Journal of industrial microbiology & biotechnology · 2017Article
- Hxt13, Hxt15, Hxt16 and Hxt17 from Saccharomyces cerevisiae represent a novel type of polyol transporters.Scientific reports · 2016Article
- Transcriptional analysis of Kluyveromyces marxianus for ethanol production from inulin using consolidated bioprocessing technology.Biotechnology for biofuels · 2015Article
- Kinetics and regulation of lactose transport and metabolism in Kluyveromyces lactis JA6.World journal of microbiology & biotechnology · 2014Article
- Extensive intra-kingdom horizontal gene transfer converging on a fungal fructose transporter gene.PLoS genetics · 2013Article
- Regulations of sugar transporters: insights from yeast.Current genetics · 2013Review
- ZrFsy1, a high-affinity fructose/H+ symporter from fructophilic yeast Zygosaccharomyces rouxii.PloS one · 2013Article
- Identification of hexose transporter-like sensor HXS1 and functional hexose transporter HXT1 in the methylotrophic yeast Hansenula polymorpha.Eukaryotic cell · 2008Article
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2 authors at 1 institution in 1 country.
Funding
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Abstract
Most yeast hexose transporters studied so far at the molecular level mediate facilitated diffusion of glucose and fructose. Here, we report that a novel Kluyveromyces lactis gene, FRT1, encodes a proton-coupled fructose-uptake transporter. Frt1, when expressed in a Saccharomyces cerevisiae hxt null mutant strain that is unable to take up monosaccharides, restored growth on fructose. Determination of substrate specificities and kinetic parameters revealed Frt1 as a fructose transporter with a K(m) of 0.16+/-0.02 mM. Uptake of fructose was accompanied by an initial alkalization of the medium, indicating a proton-coupled uptake mechanism. Deletion of the FRT1 gene in a K. lactis strain already deleted for its RAG1 and HGT1 hexose transporter genes completely prevented uptake of and growth with fructose but not with glucose. Kinetic parameters of Frt1 in K. lactis, as assessed in a rag1 hgt1 mutant strain, were comparable with those obtained after heterologous expression in S. cerevisiae. Transcription of the FRT1 gene, which was undetectable when cells were grown in ethanol, was induced by various sugars. Our results indicate that, unlike S. cerevisiae, K. lactis exhibits proton symport systems for the uptake of hexoses, in addition to their facilitated diffusion.
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