ArticleProceedings of the National Academy of Sciences of the United States of America2003
High-fidelity in vivo replication of DNA base shape mimics without Watson-Crick hydrogen bonds.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 76 citations in OpenAlex.
- Complementary base pair interactions between different rare tautomers of the second-generation artificial genetic alphabets.Journal of molecular modeling · 2023Article
- Establishing Linkages Among DNA Damage, Mutagenesis, and Genetic Diseases.Chemical research in toxicology · 2022Review
- Translation of non-standard codon nucleotides reveals minimal requirements for codon-anticodon interactions.Nature communications · 2018Article
- Evolution of functional six-nucleotide DNA.Journal of the American Chemical Society · 2015Article
- In vitro selection with artificial expanded genetic information systems.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Measurement and theory of hydrogen bonding contribution to isosteric DNA base pairs.Journal of the American Chemical Society · 2012Article
- Amplification, mutation, and sequencing of a six-letter synthetic genetic system.Journal of the American Chemical Society · 2011Article
- Non-natural nucleotides as probes for the mechanism and fidelity of DNA polymerases.Biochimica et biophysica acta · 2010Review
- DNA damage and interstrand cross-link formation upon irradiation of aryl iodide C-nucleotide analogues.The Journal of organic chemistry · 2010Article
- pH-Dependent configurations of a 5-chlorouracil-guanine base pair.Biochemistry · 2009Article
- A man-made ATP-binding protein evolved independent of nature causes abnormal growth in bacterial cells.PloS one · 2009Article
- Amino acid architecture that influences dNTP insertion efficiency in Y-family DNA polymerase V of E. coli.Journal of molecular biology · 2009Article
- Redesigning the architecture of the base pair: toward biochemical and biological function of new genetic sets.Chemistry & biology · 2009Review
- An unnatural base pair system for efficient PCR amplification and functionalization of DNA molecules.Nucleic acids research · 2009Article
- Unnatural substrate repertoire of A, B, and X family DNA polymerases.Journal of the American Chemical Society · 2008Article
- DNA polymerase bypass in vitro and in E. coli of a C-nucleotide analogue of Fapy-dG.Bioorganic & medicinal chemistry · 2008Article
- Biological properties of single chemical-DNA adducts: a twenty year perspective.Chemical research in toxicology · 2008Review
- Crystal structure, stability and in vitro RNAi activity of oligoribonucleotides containing the ribo-difluorotoluyl nucleotide: insights into substrate requirements by the human RISC Ago2 enzyme.Nucleic acids research · 2007Article
- Probing the active site tightness of DNA polymerase in subangstrom increments.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Polymerase evolution: efforts toward expansion of the genetic code.Journal of the American Chemical Society · 2005Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
We report studies testing the importance of Watson-Crick hydrogen bonding, base-pair geometry, and steric effects during DNA replication in living bacterial cells. Nonpolar DNA base shape mimics of thymine and adenine (abbreviated F and Q, respectively) were introduced into Escherichia coli by insertion into a phage genome followed by transfection of the vector into bacteria. Genetic assays showed that these two base mimics were bypassed with moderate to high efficiency in the cells and with very high efficiency under damage-response (SOS induction) conditions. Under both sets of conditions, the T-shape mimic (F) encoded genetic information in the bacteria as if it were thymine, directing incorporation of adenine opposite it with high fidelity. Similarly, the A mimic (Q) directed incorporation of thymine opposite itself with high fidelity. The data establish that Watson-Crick hydrogen bonding is not necessary for high-fidelity replication of a base pair in vivo. The results suggest that recognition of DNA base shape alone serves as the most powerful determinant of fidelity during transfer of genetic information in a living organism.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.