ArticleApplied and environmental microbiology2003
Direct profiling of environmental microbial populations by thermal dissociation analysis of native rRNAs hybridized to oligonucleotide microarrays.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Effect of a Flaxseed Lignan Intervention on Circulating Bile Acids in a Placebo-Controlled Randomized, Crossover Trial.Nutrients · 2020Trial
- Modeling formamide denaturation of probe-target hybrids for improved microarray probe design in microbial diagnostics.PloS one · 2012Article
- High-throughput analysis of ammonia oxidiser community composition via a novel, amoA-based functional gene array.PloS one · 2012Article
- Direct rRNA fingerprinting, a novel method to profile low diversity microbial communities.Microbial ecology · 2011Article
- Microarray-based evaluation of whole-community genome DNA amplification methods.Applied and environmental microbiology · 2011Article
- Quantitative rRNA-targeted solution-based hybridization assay using peptide nucleic acid molecular beacons.Applied and environmental microbiology · 2008Article
- In situ-synthesized virulence and marker gene biochip for detection of bacterial pathogens in water.Applied and environmental microbiology · 2008Article
- Prokaryotic lifestyles in deep sea habitats.Extremophiles : life under extreme conditions · 2008Review
- Unravelling microbial communities with DNA-microarrays: challenges and future directions.Microbial ecology · 2007Review
- Influence of dangling ends and surface-proximal tails of targets on probe-target duplex formation in 16S rRNA gene-based diagnostic arrays.Applied and environmental microbiology · 2007Article
- Revision of the nonequilibrium thermal dissociation and stringent washing approaches for identification of mixed nucleic acid targets by microarrays.Nucleic acids research · 2007Article
- Microarray-based analysis of microbial community RNAs by whole-community RNA amplification.Applied and environmental microbiology · 2007Article
- Microarray applications in microbial ecology research.Microbial ecology · 2006Review
- A DNA microarray platform based on direct detection of rRNA for characterization of freshwater sediment-related prokaryotic communities.Applied and environmental microbiology · 2006Article
- Suspension array analysis of 16S rRNA from Fe- and SO(4)2- reducing bacteria in uranium-contaminated sediments undergoing bioremediation.Applied and environmental microbiology · 2006Article
- Microarray-based analysis of subnanogram quantities of microbial community DNAs by using whole-community genome amplification.Applied and environmental microbiology · 2006Article
- Technology development to explore the relationship between oral health and the oral microbial community.BMC oral health · 2006Article
- On-chip non-equilibrium dissociation curves and dissociation rate constants as methods to assess specificity of oligonucleotide probes.Nucleic acids research · 2006Article
- Rapid quantitative profiling of complex microbial populations.Nucleic acids research · 2006Article
- Evaluation of gel-pad oligonucleotide microarray technology by using artificial neural networks.Applied and environmental microbiology · 2005Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oligonucleotide microarrays were used to profile directly extracted rRNA from environmental microbial populations without PCR amplification. In our initial inspection of two distinct estuarine study sites, the hybridization patterns were reproducible and varied between estuarine sediments of differing salinities. The determination of a thermal dissociation curve (i.e., melting profile) for each probe-target duplex provided information on hybridization specificity, which is essential for confirming adequate discrimination between target and nontarget sequences.
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