ArticleApplied and environmental microbiology2003
PCR-restriction fragment length polymorphism analysis of the phospholipase B (PLB1) gene for subtyping of Cryptococcus neoformans isolates.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Molecular epidemiological investigation ofMicrobiology spectrum · 2024Article
- Molecular Markers Reveal Epidemiological Patterns and Evolutionary Histories of the Human PathogenicFrontiers in cellular and infection microbiology · 2021Review
- A Glucuronoxylomannan Epitope Exhibits Serotype-Specific Accessibility and Redistributes towards the Capsule Surface during Titanization of the Fungal Pathogen Cryptococcus neoformans.Infection and immunity · 2019Article
- Genotypic Diversity Is Independent of Pathogenicity in Colombian Strains ofJournal of fungi (Basel, Switzerland) · 2018Article
- Cryptococcus gattii infections.Clinical microbiology reviews · 2014Review
- Molecular Epidemiology of Clinical Cryptococcus neoformans Isolates in Seoul, Korea.Mycobiology · 2014Article
- Global Molecular Epidemiology of Cryptococcus neoformans and Cryptococcus gattii: An Atlas of the Molecular Types.Scientifica · 2013Review
- Molecular epidemiology reveals genetic diversity amongst isolates of the Cryptococcus neoformans/C. gattii species complex in Thailand.PLoS neglected tropical diseases · 2013Article
- Cryptococcus gattii infection in an immunocompetent patient from Southern Italy.Mycopathologia · 2012Article
- Genetic diversity of the Cryptococcus species complex suggests that Cryptococcus gattii deserves to have varieties.PloS one · 2009Article
- Clinical presentation, diagnosis and management of Cryptococcus gattii cases: Lessons learned from British Columbia.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2009Article
- Consensus multi-locus sequence typing scheme for Cryptococcus neoformans and Cryptococcus gattii.Medical mycology · 2009Review
- Identification of genotypically diverse Cryptococcus neoformans and Cryptococcus gattii isolates by Luminex xMAP technology.Journal of clinical microbiology · 2007Article
- Use of a suspension array for rapid identification of the varieties and genotypes of the Cryptococcus neoformans species complex.Journal of clinical microbiology · 2005Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cryptococcus neoformans is a pathogenic yeast that is currently divided into three varieties, five serotypes, and eight molecular types. The following report describes the use of PCR-restriction fragment length polymorphism (RFLP) analysis of the phospholipase B gene (PLB1) as a simple tool to differentiate between C. neoformans subgroups. A PLB1 fragment, 1,970 bp, was amplified and digested with either AvaI or HindIII. Both sets of profiles grouped the isolates into their respective varieties, but only the AvaI profiles allowed for the identification of the eight molecular types via the corresponding RFLP profiles A1 to A8. Digestion of the same fragments with HindIII resulted in RFLP profiles H1 to H5, which distinguished only between serotype A, AD, D, and B/C. Neither enzyme distinguished serotype B from serotype C. The serotype AD profile was a composite of the serotype A and D profiles. Further investigation showed that the serotype AD isolates used in this study are heterozygous, with one allele of PLB1 originating from a serotype A parent and the other from a serotype D parent.
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