ArticleApplied and environmental microbiology2003
Evolutionary engineering of Saccharomyces cerevisiae for anaerobic growth on xylose.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 247 citations in OpenAlex.
- FromJournal of fungi (Basel, Switzerland) · 2023Review
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- Recent advances in the microbial production of squalene.World journal of microbiology & biotechnology · 2022Review
- Reversible amyloids of pyruvate kinase couple cell metabolism and stress granule disassembly.Nature cell biology · 2021Article
- Genomics and transcriptomics analysis reveals the mechanism of isobutanol tolerance of a laboratory evolved Lactococcus lactis strain.Scientific reports · 2020Article
- Current Status and Applications of Adaptive Laboratory Evolution in Industrial Microorganisms.Journal of microbiology and biotechnology · 2020Review
- Metabolomics Analysis Reveals Global Metabolic Changes in the EvolvedMetabolites · 2020Article
- High-Efficiency Biocatalytic Conversion of Thebaine to Codeine.ACS omega · 2020Article
- Metabolic engineering considerations for the heterologous expression of xylose-catabolic pathways in Saccharomyces cerevisiae.PloS one · 2020Article
- The emergence of adaptive laboratory evolution as an efficient tool for biological discovery and industrial biotechnology.Metabolic engineering · 2019Review
- Article
- Cell Aggregation and Aerobic Respiration Are Important forApplied and environmental microbiology · 2019Article
- Extracellular Phytase Production by the Wine Yeast S. cerevisiae (Finarome Strain) during Submerged Fermentation.Molecules (Basel, Switzerland) · 2018Article
- Metabolic engineering of a xylose pathway for biotechnological production of glycolate in Escherichia coli.Microbial cell factories · 2018Article
- Understanding low radiation background biology through controlled evolution experiments.Evolutionary applications · 2017Article
- Saccharomyces cerevisiae strains for second-generation ethanol production: from academic exploration to industrial implementation.FEMS yeast research · 2017Review
- Engineering tolerance to industrially relevant stress factors in yeast cell factories.FEMS yeast research · 2017Review
- Article
- Unraveling the genetic basis of xylose consumption in engineered Saccharomyces cerevisiae strains.Scientific reports · 2016Article
- Xylose Fermentation by Saccharomyces cerevisiae: Challenges and Prospects.International journal of molecular sciences · 2016Review
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Authors and funding
2 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Xylose utilization is of commercial interest for efficient conversion of abundant plant material to ethanol. Perhaps the most important ethanol-producing organism, Saccharomyces cerevisiae, however, is incapable of xylose utilization. While S. cerevisiae strains have been metabolically engineered to utilize xylose, none of the recombinant strains or any other naturally occurring yeast has been able to grow anaerobically on xylose. Starting with the recombinant S. cerevisiae strain TMB3001 that overexpresses the xylose utilization pathway from Pichia stipitis, in this study we developed a selection procedure for the evolution of strains that are capable of anaerobic growth on xylose alone. Selection was successful only when organisms were first selected for efficient aerobic growth on xylose alone and then slowly adapted to microaerobic conditions and finally anaerobic conditions, which indicated that multiple mutations were necessary. After a total of 460 generations or 266 days of selection, the culture reproduced stably under anaerobic conditions on xylose and consisted primarily of two subpopulations with distinct phenotypes. Clones in the larger subpopulation grew anaerobically on xylose and utilized both xylose and glucose simultaneously in batch culture, but they exhibited impaired growth on glucose. Surprisingly, clones in the smaller subpopulation were incapable of anaerobic growth on xylose. However, as a consequence of their improved xylose catabolism, these clones produced up to 19% more ethanol than the parental TMB3001 strain produced under process-like conditions from a mixture of glucose and xylose.
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