ArticleJournal of perinatology : official journal of the California Perinatal Association2003
Detection of fungemia by polymerase chain reaction in critically ill neonates and children.
Article in Journal of perinatology : official journal of the California Perinatal Association, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 3 of them syntheses that pooled it.
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Who cites it
14 citing papers in PubMed, 3 syntheses or guidelines pooled it, 72 citations in OpenAlex.
- Molecular assays for the diagnosis of sepsis in neonates: a diagnostic test accuracy review.The Cochrane database of systematic reviews · 2025Pooled it
- Molecular assays for the diagnosis of sepsis in neonates.The Cochrane database of systematic reviews · 2017Pooled it
- PCR diagnosis of invasive candidiasis: systematic review and meta-analysis.Journal of clinical microbiology · 2011Pooled it
- Syndrome Evaluation System (SES) versus Blood Culture (BACTEC) in the Diagnosis and Management of Neonatal Sepsis--A Randomized Controlled Trial.Indian journal of pediatrics · 2016Trial
- First case of neonatal bloodstream infection caused byExperimental and therapeutic medicine · 2026Article
- Article
- Utility of Panfungal PCR in the diagnosis of invasive fungal infections in febrile neutropenia.Journal of family medicine and primary care · 2021Article
- Progress in the Diagnosis of Invasive Fungal Disease in Children.Current fungal infection reports · 2017Review
- A multiplex nested PCR for the detection and identification of Candida species in blood samples of critically ill paediatric patients.BMC infectious diseases · 2014Article
- Molecular and nonmolecular diagnostic methods for invasive fungal infections.Clinical microbiology reviews · 2014Review
- Polymerase chain reaction-based assays for the diagnosis of invasive fungal infections.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2012Review
- Molecular microbiological methods in the diagnosis of neonatal sepsis.Expert review of anti-infective therapy · 2010Review
- PCR-based diagnosis of neonatal staphylococcal bacteremias.Journal of clinical microbiology · 2005Article
- Clinical microbiology of bacterial and fungal sepsis in very-low-birth-weight infants.Clinical microbiology reviews · 2004Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo compare polymerase chain reaction (PCR) with blood culture for the detection of fungemia in neonatal and pediatric intensive care unit patients. STUDY
designA total of 0.2 to 0.5 ml of blood was collected simultaneously with blood culture in patients with suspected sepsis. Following DNA extraction, the gene for fungal 18S rRNA was amplified and PCR products analyzed by agarose gel electrophoresis.
resultsOf 70 patient samples, nine of nine with a positive blood culture for Candida were PCR positive, and one of four with a positive blood culture for Malassezia was PCR positive. In total, 13 of 57 samples with negative blood culture for fungus were PCR positive. Seven of these 13 patients had other evidence of invasive fungal disease. In contrast, none of the 44 blood culture-negative/PCR-negative patients had other evidence of fungal infection.
conclusionsPCR may be a useful adjunct to blood culture for the rapid detection of fungemia in high-risk patients.
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