Evidence map›Paper›PMID 12672412›Full record

ArticleAnalytical biochemistry2003

Effects of fluorophore-to-silver distance on the emission of cyanine-dye-labeled oligonucleotides.

Joanna Malicka, Ignacy Gryczynski, Zygmunt Gryczynski, Joseph R Lakowicz

Open access · greenAbstract readComparative Study
In one paragraph

Article in Analytical biochemistry, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.

0numbers the graph read from it
0cells of the map it votes in
62citing papers in PubMed
9.5field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

62 citing papers in PubMed, 200 citations in OpenAlex.

  1. Plasmon- and Waveguide-Coupled Fluorescence at the Ultraviolet Region.The journal of physical chemistry. C, Nanomaterials and interfaces · 2023
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  2. Silica Shell Thickness-Dependent Fluorescence Properties of SiOInternational journal of molecular sciences · 2022
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  3. Metal-Enhanced HgACS omega · 2022
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  4. Review
  5. Dansyl-Labelled Ag@SiOMaterials (Basel, Switzerland) · 2020
    Article
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  10. Facile Functionalization of Ag@SiOAnalytical methods : advancing methods and applications · 2014
    Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Fluorescence enhancement using silver-gold nanocomposite substrates.Proceedings of SPIE--the International Society for Optical Engineering · 2012
    Article
  16. Review
  17. Article
  18. Article
  19. Article
  20. Article

2 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Joanna MalickaDepartment of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
Ignacy Gryczynski
Zygmunt Gryczynski
Joseph R Lakowicz
University of Maryland, Baltimore · US

Funding

WHO'S WHO IN FLUORESCENCEP41RR008119 · NCRR · UNIVERSITY OF MARYLAND BALTIMORE · PI BASKAKOV, ILIA V · 1993 to 2006
$7.4M
Metal-Enhanced Fluorescence SensingR01EB000682 · NIBIB · UNIVERSITY OF MARYLAND BALTIMORE · PI LAKOWICZ, JOSEPH R. · 2002 to 2006
$1.7M
NCRR NIH HHS P41 RR008119NCRR NIH HHS RR-08119NIBIB NIH HHS EB000682NIBIB NIH HHS R01 EB000682
6 · The paper itself

Abstract

We examined the fluorescence spectral properties of Cy3- and Cy5-labeled oligonucleotides at various distances from the surface of silver island films. The distance to the surface was controlled by alternating layers of biotinylated bovine serum albumin (BSA) and avidin, followed by binding of a biotinylated oligonucleotide. The maximum enhancement of fluorescence near a factor of 12 was observed for the first BSA-avidin layer, with the enhancement decreasing to 2-fold for six layers. The minimum lifetimes were observed for the first BSA-avidin layer, and were about 25-fold shorter than on quartz slides without silver, with the lifetimes being about 2-fold shorter for six BSA-avidin layers. These results suggest that maximum fluorescence enhancements occur about 90A from the silver surface, a distance readily obtained by one or two layers of proteins.

Indexed as

AnimalsAvidinBase SequenceBiotinCarbocyaninesCattleColloidsDNAFluorescent DyesHalf-LifeMicroscopy, Atomic ForceOligonucleotidesQuartzSerum Albumin, BovineSilverSpectrometry, FluorescenceAvidinBiotinCarbocyaninesColloidscyanine dye 3cyanine dye 5DNAFluorescent DyesOligonucleotidesQuartzSerum Albumin, BovineSilver

Identifiers

PMID12672412
PMCPMC2753827
OpenAlexW2080664554

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.