ArticleEMBO reports2003
ORFB is a subunit of F1F(O)-ATP synthase: insight into the basis of cytoplasmic male sterility in sunflower.
Article in EMBO reports, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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Who cites it
68 citing papers in PubMed, 190 citations in OpenAlex.
- 3-Hydroxypropanoic acid contributing to pollen abortion in ogura cytoplasmic male sterility of Brassica napus.BMC plant biology · 2026Article
- Past and future of cytoplasmic male sterility and heterosis breeding in crop plants.Plant cell reports · 2025Review
- Insights into cellular crosstalk regulating cytoplasmic male sterility and fertility restoration.Molecular biology reports · 2024Review
- Characterization of the CMS genetic regulation through comparative complete mitochondrial genome sequencing in Nicotiana tabacum.The plant genome · 2024Article
- Potential candidate genes and pathways related to cytoplasmic male sterility in Dianthus spiculifolius as revealed by transcriptome analysis.Plant cell reports · 2023Article
- Molecular genetics of cytoplasmic male sterility and restorer-of-fertility for the fine tuning of pollen production in crops.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Review
- Current insights and advances into plant male sterility: new precision breeding technology based on genome editing applications.Frontiers in plant science · 2023Review
- Induction of Male Sterility by Targeted Mutation of a Restorer-of-Fertility Gene with CRISPR/Cas9-Mediated Genome Editing inPlants (Basel, Switzerland) · 2022Article
- Rice functional genomics: decades' efforts and roads ahead.Science China. Life sciences · 2022Review
- Effects of Different Chemicals on Sexual Regulation in Persimmon (Frontiers in plant science · 2022Article
- Comparative physiological and proteomic analyses of mangrove plant Kandelia obovata under cold stress.Ecotoxicology (London, England) · 2021Article
- Mitochondrial genes are involved in the fertility transformation of the thermosensitive male-sterile line YS3038 in wheat.Molecular breeding : new strategies in plant improvement · 2021Article
- Morphological characteristics and transcriptome analysis at different anther development stages of the male sterile mutant MS7-2 in Wucai (Brassica campestris L.).BMC genomics · 2021Article
- Combined Transcriptome and Proteome Analysis of Anthers of AL-type Cytoplasmic Male Sterile Line and Its Maintainer Line Reveals New Insights into Mechanism of Male Sterility in Common Wheat.Frontiers in genetics · 2021Article
- Unraveling the Genetic Basis of Fertility Restoration for Cytoplasmic Male Sterile LineFrontiers in plant science · 2021Article
- Orf165 is associated with cytoplasmic male sterility in pepper.Genetics and molecular biology · 2021Article
- Comparative analysis of mitochondrial genomes of soybean cytoplasmic male-sterile lines and their maintainer lines.Functional & integrative genomics · 2021Article
- The Mitochondrial Genome Assembly of Fennel (International journal of molecular sciences · 2020Article
- Comparative Transcriptome Analysis Reveals the Potential Mechanism of Abortion in TobaccoInternational journal of molecular sciences · 2020Article
- Transcriptome profiling of differentially expressed genes in cytoplasmic male-sterile line and its fertility restorer line in pigeon pea (Cajanus cajan L.).BMC plant biology · 2020Article
8 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
ORFB is the product of a gene that is conserved in plant mitochondrial genomes, and which, on the basis of sequence motif and structural similarity, is predicted to be the homologue of yeast and mammalian ATP8, part of the F(O) component of the F1F(O)-ATP synthase. We have shown that, in sunflower, orfB transcripts are edited, increasing the similarity of the predicted protein to ATP8 proteins from non-plant species. Blue-native polyacrylamide gel electrophoresis and peptide sequencing confirm that ORFB localizes to the ATP synthase complex. The predicted amino-terminal 19 amino acids of ORFB are identical to those in the chimeric mitochondrial ORF522 protein, which is associated with cytoplasmic male sterility (CMS) in sunflower. Assays comparing respiratory complexes from a male-sterile line expressing ORF522 with those from a male-fertile line show a specific decrease in ATP hydrolysis by the ATP synthase. These observations allow us to propose a mechanism underlying CMS that is associated with the expression of chimeric open reading frames containing part of the orfB gene.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.