ArticleThe Plant cell2003
Increased lysine synthesis coupled with a knockout of its catabolism synergistically boosts lysine content and also transregulates the metabolism of other amino acids in Arabidopsis seeds.
Article in The Plant cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed, 205 citations in OpenAlex.
- Novel Amino Acid Changes Increase Phosphoenolpyruvate Carboxylase Output in planta.Plant, cell & environment · 2026Article
- Mitochondrial β-carbonic anhydrase is a conserved metabolic rheostat for branched-chain amino acid catabolism and metabolic flexibility.The New phytologist · 2026Article
- Lysine-rich rice enhanced muscle growth and development in young rats.European journal of nutrition · 2025Article
- New Insights into the Genetic Basis of Lysine Accumulation in Rice Revealed by Multi-Model GWAS.International journal of molecular sciences · 2024Article
- Article
- OsbZIP18 Is a Positive Regulator of Phenylpropanoid and Flavonoid Biosynthesis under UV-B Radiation in Rice.Plants (Basel, Switzerland) · 2024Article
- UV-B Irradiation to Amino Acids and Carbohydrate Metabolism inPlants (Basel, Switzerland) · 2022Article
- The Delay ofPlants (Basel, Switzerland) · 2022Article
- Engineering a feedback inhibition-insensitive plant dihydrodipicolinate synthase to increase lysine content in Camelina sativa seeds.Transgenic research · 2022Article
- Genome-wide association study of traits in sacred lotus uncovers MITE-associated variants underlying stamen petaloid and petal number variations.Frontiers in plant science · 2022Article
- l-Aspartate: An Essential Metabolite for Plant Growth and Stress Acclimation.Molecules (Basel, Switzerland) · 2021Review
- Novel Transcriptome Study and Detection of Metabolic Variations in UV-B-Treated Date Palm (International journal of molecular sciences · 2021Article
- Lysine biofortification in rice by modulating feedback inhibition of aspartate kinase and dihydrodipicolinate synthase.Plant biotechnology journal · 2021Article
- Unraveling the Genetic Basis of Fertility Restoration for Cytoplasmic Male Sterile LineFrontiers in plant science · 2021Article
- Article
- An iron (II) dependent oxygenase performs the last missing step of plant lysine catabolism.Nature communications · 2020Article
- Utilization of GC-MS untargeted metabolomics to assess the delayed response of glufosinate treatment of transgenic herbicide resistant (HR) buffalo grasses (Stenotaphrum secundatum L.).Metabolomics : Official journal of the Metabolomic Society · 2020Article
- Lysine, Lysine-Rich, Serine, and Serine-Rich Proteins: Link Between Metabolism, Development, and Abiotic Stress Tolerance and the Role of ncRNAs in Their Regulation.Frontiers in plant science · 2020Review
- An L,L-diaminopimelate aminotransferase mutation leads to metabolic shifts and growth inhibition in Arabidopsis.Journal of experimental botany · 2018Article
- New insights into the metabolism of aspartate-family amino acids in plant seeds.Plant reproduction · 2018Review
4 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To elucidate the relative significance of Lys synthesis and catabolism in determining Lys level in plant seeds, we expressed a bacterial feedback-insensitive dihydrodipicolinate synthase (DHPS) in a seed-specific manner in wild-type Arabidopsis as well as in an Arabidopsis knockout mutant in the Lys catabolism pathway. Transgenic plants expressing the bacterial DHPS, or the knockout mutant, contained approximately 12-fold or approximately 5-fold higher levels, respectively, of seed free Lys than wild-type plants. However, the combination of these two traits caused a synergistic approximately 80-fold increase in seed free Lys level. The dramatic increase in free Lys in the knockout mutant expressing the bacterial DHPS was associated with a significant reduction in the levels of Glu and Asp but also with an unexpected increase in the levels of Gln and Asn. This finding suggested a special regulatory interaction between Lys metabolism and amide amino acid metabolism in seeds. Notably, the level of free Met, which competes with Lys for Asp and Glu as precursors, was increased unexpectedly by up to approximately 38-fold in the various transgenic and knockout plants. Together, our results show that Lys catabolism plays a major regulatory role in Lys accumulation in Arabidopsis seeds and reveal novel regulatory networks of seed amino acid metabolism.
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