ArticleProceedings of the National Academy of Sciences of the United States of America2003
Protein trans-splicing in transgenic plant chloroplast: reconstruction of herbicide resistance from split genes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 58 citations in OpenAlex.
- Extein residues regulate the catalytic function of Spl DnaX intein enzyme by restricting the near-attack conformations of the active-site residues.Protein science : a publication of the Protein Society · 2023Article
- Inteins in Science: Evolution to Application.Microorganisms · 2020Review
- The application of gene splitting technique for controlling transgene flow in rice.Transgenic research · 2020Article
- Biotechnological Applications of Protein Splicing.Current protein & peptide science · 2019Review
- Tolerance of Transplastomic Tobacco Plants Overexpressing a Theta Class Glutathione Transferase to Abiotic and Oxidative Stresses.Frontiers in plant science · 2018Article
- Intein applications: from protein purification and labeling to metabolic control methods.The Journal of biological chemistry · 2014Review
- Article
- Gene-splitting technology: a novel approach for the containment of transgene flow in Nicotiana tabacum.PloS one · 2014Article
- The production of male-sterile wheat plants through split barnase expression is promoted by the insertion of introns and flexible peptide linkers.Transgenic research · 2013Article
- Recent progress in intein research: from mechanism to directed evolution and applications.Cellular and molecular life sciences : CMLS · 2013Review
- Engineering a thermoregulated intein-modified xylanase into maize for consolidated lignocellulosic biomass processing.Nature biotechnology · 2012Article
- Various mechanisms in cyclopeptide production from precursors synthesized independently of non-ribosomal peptide synthetases.Acta biochimica et biophysica Sinica · 2011Review
- Traceless protein splicing utilizing evolved split inteins.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Reconstitution of glyphosate resistance from a split 5-enolpyruvyl shikimate-3-phosphate synthase gene in Escherichia coli and transgenic tobacco.Applied and environmental microbiology · 2007Article
- Functional analysis of the split Synechocystis DnaE intein in plant tissues by biolistic particle bombardment.Transgenic research · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Inteins are intervening protein sequences that undergo self-excision from a precursor protein with concomitant joining of the flanking sequences. Here, we demonstrate intein trans-splicing in Nicotiana tabacum chloroplasts by using the naturally split Ssp DnaE intein. Trans-splicing occurred whether both intein fragments were encoded in the chloroplast or were separated into the chloroplast and nuclear genomes. A biolistic approach was used to integrate two fusion genes, one encoding aminoglycoside-3-adenyltransferase (aadA) and the first 123 aa of the Ssp DnaE intein (In) and the other encoding 36 C-terminal amino acid residues of the Ssp DnaE intein (Ic) and soluble modified green fluorescent protein (smGFP) into N. tabacum plastids. Expression of these gene fragments in the chloroplast resulted in ligated aadA-smGFP due to In-Ic-mediated trans-splicing. Furthermore, an N-terminal portion of the herbicide resistance gene 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) containing a chloroplast localization signal fused to In (EPSPSn-In) was integrated into the nuclear DNA of N. tabacum by using Agrobacterium tumefaciens-mediated transformation. The remaining EPSPS gene fragment (EPSPSc) fused to Ic (Ic-EPSPSc) was integrated into the chloroplast genome by homologous recombination. Western blot analysis of cell extracts from these plants showed a full-length EPSPS, demonstrating that the EPSPSn-In gene product migrated to the chloroplast and underwent trans-splicing. Furthermore, these transgenic plants displayed improved resistance to the herbicide N-(phosphonomethyl)glycine (glyphosate) when compared with wild-type N. tabacum.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.