ArticleJournal of bacteriology2003
In vitro properties of RpoS (sigma(S)) mutants of Escherichia coli with postulated N-terminal subregion 1.1 or C-terminal region 4 deleted.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Article
- RpoS impacts global gene expression and carbon source utilization inFrontiers in microbiology · 2025Article
- The dormancy-specific regulator, SutA, is intrinsically disordered and modulates transcription initiation in Pseudomonas aeruginosa.Molecular microbiology · 2019Article
- Regulation of acid resistance by connectors of two-component signal transduction systems in Escherichia coli.Journal of bacteriology · 2011Article
- Identification of conserved amino acid residues of the Salmonella sigmaS chaperone Crl involved in Crl-sigmaS interactions.Journal of bacteriology · 2010Article
- E Unibus Plurum: genomic analysis of an experimentally evolved polymorphism in Escherichia coli.PLoS genetics · 2009Article
- Binding of the unorthodox transcription activator, Crl, to the components of the transcription machinery.The Journal of biological chemistry · 2008Article
- Poising of Escherichia coli RNA polymerase and its release from the sigma 38 C-terminal tail for osmY transcription.Journal of molecular biology · 2008Article
- Limited role for the DsrA and RprA regulatory RNAs in rpoS regulation in Salmonella enterica.Journal of bacteriology · 2006Article
- A stop codon-dependent internal secondary translation initiation region in Escherichia coli rpoS.RNA (New York, N.Y.) · 2004Article
- Properties of Bacillus subtilis sigma A factors with region 1.1 and the conserved Arg-103 at the N terminus of region 1.2 deleted.Journal of bacteriology · 2004Article
- A comparative study of variation in codon 33 of the rpoS gene in Escherichia coli K12 stocks: implications for the synthesis of sigma(s).Molecular genetics and genomics : MGG · 2004Article
- Error-prone DNA polymerase IV is controlled by the stress-response sigma factor, RpoS, in Escherichia coli.Molecular microbiology · 2003Article
Corrections and comments
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Authors and funding
4 authors.
Funding
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Abstract
Derivatives of the stationary-phase sigma factor sigma(S) of Escherichia coli lacking either of two conserved domains, the postulated N-terminal subregion 1.1 or the C-terminal region 4, were shown to be competent in vitro for transcription initiation from several sigma(S)-dependent promoters on supercoiled DNA templates. Unlike wild-type sigma(S), however, the deletion derivatives were inactive on relaxed templates. The anomalous slow electrophoretic mobility of sigma(S) on denaturing gels was corrected by deletion of subregion 1.1, suggesting that this domain in sigma(S) may be structurally and functionally analogous to subregion 1.1 of sigma(70), substitutions in which have previously been shown to rectify the anomalous electrophoretic migration of sigma(70) (V. Gopal and D. Chatterji, Eur. J. Biochem. 244:614-618, 1997).
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