ArticleJournal of bacteriology2003
Phenotypic mutants of the intracellular actinomycete Rhodococcus equi created by in vivo Himar1 transposon mutagenesis.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 59 citations in OpenAlex.
- Article
- Identification of genes required for the fitness of Rhodococcus equi during the infection of mice via signature-tagged transposon mutagenesis.The Journal of veterinary medical science · 2021Article
- Biostatistical prediction of genes essential for growth of Anaplasma phagocytophilum in a human promyelocytic cell line using a random transposon mutant library.Pathogens and disease · 2021Article
- Creating a Library of Random Transposon Mutants in Leptospira.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Discovery ofFrontiers in cellular and infection microbiology · 2020Article
- MAIT, MR1, microbes and riboflavin: a paradigm for the co-evolution of invariant TCRs and restricting MHCI-like molecules?Immunogenetics · 2016Review
- Article
- Biodegradation of the organic disulfide 4,4'-dithiodibutyric acid by Rhodococcus spp.Applied and environmental microbiology · 2015Article
- Potential mechanisms of attenuation for rifampicin-passaged strains of Flavobacterium psychrophilum.BMC microbiology · 2015Article
- Diversion of phagosome trafficking by pathogenic Rhodococcus equi depends on mycolic acid chain length.Cellular microbiology · 2013Article
- Article
- Conjugal transfer of a virulence plasmid in the opportunistic intracellular actinomycete Rhodococcus equi.Journal of bacteriology · 2012Article
- The steroid catabolic pathway of the intracellular pathogen Rhodococcus equi is important for pathogenesis and a target for vaccine development.PLoS pathogens · 2011Article
- Characterization of the role of the pathogenicity island and vapG in the virulence of the intracellular actinomycete pathogen Rhodococcus equi.Infection and immunity · 2010Article
- Transposition of fly mariner elements into bacteria as a genetic tool for mutagenesis.Genetica · 2010Review
- New vector system for random, single-step integration of multiple copies of DNA into the Rhodococcus genome.Applied and environmental microbiology · 2010Article
- Article
- Identification of two proteins that interact with the Erp virulence factor from Mycobacterium tuberculosis by using the bacterial two-hybrid system.BMC molecular biology · 2009Article
- In vivo Himar1 transposon mutagenesis of Burkholderia pseudomallei.Applied and environmental microbiology · 2008Article
- Site-specific integration of Streptomyces PhiC31 integrase-based vectors in the chromosome of Rhodococcus equi.FEMS microbiology letters · 2008Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rhodococcus equi is a facultative intracellular opportunistic pathogen of immunocompromised people and a major cause of pneumonia in young horses. An effective live attenuated vaccine would be extremely useful in the prevention of R. equi disease in horses. Toward that end, we have developed an efficient transposon mutagenesis system that makes use of a Himar1 minitransposon delivered by a conditionally replicating plasmid for construction of R. equi mutants. We show that Himar1 transposition in R. equi is random and needs no apparent consensus sequence beyond the required TA dinucleotide. The diversity of the transposon library was demonstrated by the ease with which we were able to screen for auxotrophs and mutants with pigmentation and capsular phenotypes. One of the pigmentation mutants contained an insertion in a gene encoding phytoene desaturase, an enzyme of carotenoid biosynthesis, the pathway necessary for production of the characteristic salmon color of R. equi. We identified an auxotrophic mutant with a transposon insertion in the gene encoding a putative dual-functioning GTP cyclohydrolase II-3,4-dihydroxy-2-butanone-4-phosphate synthase, an enzyme essential for riboflavin biosynthesis. This mutant cannot grow in minimal medium in the absence of riboflavin supplementation. Experimental murine infection studies showed that, in contrast to wild-type R. equi, the riboflavin-requiring mutant is attenuated because it is unable to replicate in vivo. The mutagenesis methodology we have developed will allow the characterization of R. equi virulence mechanisms and the creation of other attenuated strains with vaccine potential.
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