ArticleJournal of bacteriology2003
Transcription of Clostridium cellulovorans cellulosomal cellulase and hemicellulase genes.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
- Fungal-bacterial interaction networks in lignocellulose degradation: an integrated ecological and mechanistic perspective.Biotechnology for biofuels and bioproducts · 2026Review
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- Combined pretreatment of sugarcane bagasse using alkali and ionic liquid to increase hemicellulose content and xylanase production.BMC biotechnology · 2020Article
- Substrate-Related Factors Affecting Cellulosome-Induced Hydrolysis for Lignocellulose Valorization.International journal of molecular sciences · 2019Review
- Revisiting the Regulation of the Primary Scaffoldin Gene in Clostridium thermocellum.Applied and environmental microbiology · 2017Article
- Cellulosome stoichiometry in Clostridium cellulolyticum is regulated by selective RNA processing and stabilization.Nature communications · 2015Article
- Complex expression of the cellulolytic transcriptome of Saccharophagus degradans.Applied and environmental microbiology · 2011Article
- The complete genome sequence of Fibrobacter succinogenes S85 reveals a cellulolytic and metabolic specialist.PloS one · 2011Article
- Detection and quantification of functional genes of cellulose- degrading, fermentative, and sulfate-reducing bacteria and methanogenic archaea.Applied and environmental microbiology · 2010Article
- Lactobacillus plantarum gene clusters encoding putative cell-surface protein complexes for carbohydrate utilization are conserved in specific gram-positive bacteria.BMC genomics · 2006Article
- Transcriptional analysis of the cip-cel gene cluster from Clostridium cellulolyticum.Journal of bacteriology · 2006Article
- Molecular cloning and transcriptional and expression analysis of engO, encoding a new noncellulosomal family 9 enzyme, from Clostridium cellulovorans.Journal of bacteriology · 2005Article
- Cellulase, clostridia, and ethanol.Microbiology and molecular biology reviews : MMBR · 2005Review
- Regulation of cellulase synthesis in batch and continuous cultures of Clostridium thermocellum.Journal of bacteriology · 2005Article
- Isolation and expression of the xynB gene and its product, XynB, a consistent component of the Clostridium cellulovorans cellulosome.Journal of bacteriology · 2004Article
- Regulation of expression of cellulosomes and noncellulosomal (hemi)cellulolytic enzymes in Clostridium cellulovorans during growth on different carbon sources.Journal of bacteriology · 2004Article
- Cellulosomes from mesophilic bacteria.Journal of bacteriology · 2003Review
- Regulation of expression of cellulosomal cellulase and hemicellulase genes in Clostridium cellulovorans.Journal of bacteriology · 2003Article
- Regulation of expression of scaffoldin-related genes in Clostridium thermocellum.Journal of bacteriology · 2003Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcription of the cellulosomal cellulase/hemicellulase genes of Clostridium cellulovorans has been investigated by Northern blot, reverse transcriptase PCR (RT-PCR), primer extension, and S1 nuclease analysis. Northern hybridizations revealed that the cellulosomal cbpA gene cluster is transcribed as polycistronic mRNAs of 8 and 12 kb. The 8-kb mRNA coded for cbpA and exgS, and the 12-kb mRNA coded for cbpA, exgS, engH, and engK. The sizes of the mRNAs were about 3 kb for engE, 1.8 kb for manA, 2.7 kb for xynA, and 4 kb for pelA, indicating monocistronic transcription of these genes. Primer extension and S1 nuclease analysis of C. cellulovorans RNA showed that the transcriptional start sites of cbpA, engE, manA, and hbpA were located 233, 97, 64, and 61 bp upstream from the first nucleotide of each of the respective translation initiation codons. Alignment of the cbpA, engE, manA, and hbpA promoter regions provided evidence for highly conserved sequences that exhibited strong similarity to the sigma(A) consensus promoter sequences of gram-positive bacteria.
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