ArticleThe Journal of cell biology2003
Determinants of [Cl-] in recycling and late endosomes and Golgi complex measured using fluorescent ligands.
Article in The Journal of cell biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 74 citations in OpenAlex.
- Epithelial plasma membrane transporters as drug targets.Physiological reviews · 2026Review
- Tunable Cytosolic Chloride Indicators for Real-Time Chloride Imaging in Live Cells.bioRxiv : the preprint server for biology · 2024Article
- Chloride ions in health and disease.Bioscience reports · 2024Review
- Two-pore channels regulate endomembrane tension to enable remodeling and resolution of phagolysosomes.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Chloride transport modulators as drug candidates.American journal of physiology. Cell physiology · 2021Review
- From Pinocytosis to Methuosis-Fluid Consumption as a Risk Factor for Cell Death.Frontiers in cell and developmental biology · 2021Review
- Quantitative Imaging of BiochemistryACS central science · 2020Review
- ClThe Journal of biological chemistry · 2020Article
- Endomembrane Tension and Trafficking.Frontiers in cell and developmental biology · 2020Review
- Article
- Fluorescent Proteins for Investigating Biological Events in Acidic Environments.International journal of molecular sciences · 2018Review
- A role for the cystic fibrosis transmembrane conductance regulator in the nitric oxide-dependent release of ClJournal of neurophysiology · 2017Article
- Article
- Rational design of a quantitative, pH-insensitive, nucleic acid based fluorescent chloride reporter.Chemical science · 2016Article
- A pH-independent DNA nanodevice for quantifying chloride transport in organelles of living cells.Nature nanotechnology · 2015Article
- Trans-species communication in the Mycobacterium tuberculosis-infected macrophage.Immunological reviews · 2015Review
- Monitoring of vacuolar-type H+ ATPase-mediated proton influx into synaptic vesicles.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Nitric oxide releases Cl(-) from acidic organelles in retinal amacrine cells.Frontiers in cellular neuroscience · 2015Article
- A model of lysosomal pH regulation.The Journal of general physiology · 2013Article
- Mycobacterium tuberculosis responds to chloride and pH as synergistic cues to the immune status of its host cell.PLoS pathogens · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Chloride concentration ([Cl-]) was measured in defined organellar compartments using fluorescently labeled transferrin, alpha2-macroglobulin, and cholera toxin B-subunit conjugated with Cl--sensitive and -insensitive dyes. In pulse-chase experiments, [Cl-] in Tf-labeled early/recycling endosomes in J774 cells was 20 mM just after internalization, increasing to 41 mM over approximately 10 min in parallel to a drop in pH from 6.91 to 6.05. The low [Cl-] just after internalization (compared with 137 mM solution [Cl-]) was prevented by reducing the interior-negative Donnan potential. [Cl-] in alpha2-macroglobulin-labeled endosomes, which enter a late compartment, increased from 28 to 58 mM at 1-45 min after internalization, whereas pH decreased from 6.85 to 5.20. Cl- accumulation was prevented by bafilomycin but restored by valinomycin. A Cl- channel inhibitor slowed endosomal acidification and Cl- accumulation by approximately 2.5-fold. [Cl-] was 49 mM and pH was 6.42 in cholera toxin B subunit-labeled Golgi complex in Vero cells; Golgi compartment Cl- accumulation and acidification were reversed by bafilomycin. Our experiments provide evidence that Cl- is the principal counter ion accompanying endosomal and Golgi compartment acidification, and that an interior-negative Donnan potential is responsible for low endosomal [Cl-] early after internalization. We propose that reduced [Cl-] and volume in early endosomes permits endosomal acidification and [Cl-] accumulation without lysis.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.