ArticleThe Journal of cell biology2003
An essential role for hGle1 nucleocytoplasmic shuttling in mRNA export.
Article in The Journal of cell biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 59 citations in OpenAlex.
- Advances in the understanding of nuclear pore complexes in human diseases.Journal of cancer research and clinical oncology · 2024Review
- Phosphorylation impacts GLE1 nuclear localization and association with DDX1.Advances in biological regulation · 2023Article
- Nuclear Pore Dysfunction in Neurodegeneration.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2022Review
- Evolution and diversification of the nuclear pore complex.Biochemical Society transactions · 2021Review
- Multiple ways to a dead end: diverse mechanisms by which ALS mutant genes induce cell death.Cell cycle (Georgetown, Tex.) · 2021Review
- Mutation Screening of theFrontiers in neuroscience · 2021Article
- Functions of Gle1 are governed by two distinct modes of self-association.The Journal of biological chemistry · 2020Article
- Nucleocytoplasmic shuttling of Gle1 impacts DDX1 at transcription termination sites.Molecular biology of the cell · 2020Article
- MAPK- and glycogen synthase kinase 3-mediated phosphorylation regulates the DEAD-box protein modulator Gle1 for control of stress granule dynamics.The Journal of biological chemistry · 2019Article
- Identification of the NovelG3 (Bethesda, Md.) · 2018Article
- Control of CNS functions by RNA-binding proteins in neurological diseases.Current pharmacology reports · 2018Article
- Nup159 Weakens Gle1 Binding to Dbp5 But Does Not Accelerate ADP Release.Journal of molecular biology · 2018Article
- Structural and functional analysis of mRNA export regulation by the nuclear pore complex.Nature communications · 2018Article
- The Impact of Post-transcriptional Control: Better Living Through RNA Regulons.Frontiers in genetics · 2018Review
- Article
- Nup42 and IPTraffic (Copenhagen, Denmark) · 2017Article
- Survival beyond the perinatal period expands the phenotypes caused by mutations in GLE1.American journal of medical genetics. Part A · 2017Article
- Review
- A role for Gle1, a regulator of DEAD-box RNA helicases, at centrosomes and basal bodies.Molecular biology of the cell · 2017Article
- Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Gle1 is required for mRNA export in yeast and human cells. Here, we report that two human Gle1 (hGle1) isoforms are expressed in HeLa cells (hGle1A and B). The two encoded proteins are identical except for their COOH-terminal regions. hGle1A ends with a unique four-amino acid segment, whereas hGle1B has a COOH-terminal 43-amino acid span. Only hGle1B, the more abundant isoform, localizes to the nuclear envelope (NE) and pore complex. To test whether hGle1 is a dynamic shuttling transport factor, we microinjected HeLa cells with recombinant hGle1 and conducted photobleaching studies of live HeLa cells expressing EGFP-hGle1. Both strategies show that hGle1 shuttles between the nucleus and cytoplasm. An internal 39-amino acid domain is necessary and sufficient for mediating nucleocytoplasmic transport. Using a cell-permeable peptide strategy, we document a role for hGle1 shuttling in mRNA export. An hGle1 shuttling domain (SD) peptide impairs the export of both total poly(A)+ RNA and the specific dihydrofolate reductase mRNA. Coincidentally, SD peptide-treated cells show decreased endogenous hGle1 localization at the NE and reduced nucleocytoplasmic shuttling of microinjected, recombinant hGle1. These findings pinpoint the first functional motif in hGle1 and link hGle1 to the dynamic mRNA export mechanism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.