ArticleThe Journal of experimental medicine2003
Identification and characterization of Helicobacter pylori genes essential for gastric colonization.
Article in The Journal of experimental medicine, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 140 papers.
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Who cites it
140 citing papers in PubMed, 266 citations in OpenAlex.
- Unveiling a missing component of the atypical type IV secretion system required for natural transformation of Helicobacter pylori.PLoS pathogens · 2026Article
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- Unveiling a missing component of the atypical type IV secretion system required for natural transformation ofbioRxiv : the preprint server for biology · 2026Article
- The causal relationship between antibody-mediated immune responses to infections and Graves' disease: a bi-directional Mendelian randomization analysis.Experimental biology and medicine (Maywood, N.J.) · 2026Article
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- Vacuolating Cytotoxin A (VacA) and Extracellular Vesicles inIranian journal of pathology · 2025Review
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- Helicobacter pylori FlgN binds its substrate FlgK and the flagellum ATPase FliI in a similar manner observed for the FliT chaperone.Protein science : a publication of the Protein Society · 2024Article
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- In vitro anti-Helicobacter pylori activity and antivirulence activity of cetylpyridinium chloride.PloS one · 2024Article
- Essential role ofInfection and immunity · 2023Article
- Proline catabolism is a key factor facilitating Candida albicans pathogenicity.PLoS pathogens · 2023Article
- DNA Transport through the Dynamic Type IV Secretion System.Infection and immunity · 2023Review
- Biochemical and Kinetic Characterization of the Glucose-6-Phosphate Dehydrogenase fromMicroorganisms · 2022Article
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- Proteolytic Landscapes in Gastric Pathology and Cancerogenesis.International journal of molecular sciences · 2022Review
- Epigenetics andInternational journal of molecular sciences · 2022Review
- Genomic differentiation within East AsianMicrobial genomics · 2022Article
80 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Helicobacter pylori causes one of the most common, chronic bacterial infections and is a primary cause of severe gastric disorders. To unravel the bacterial factors necessary for the process of gastric colonization and pathogenesis, signature tagged mutagenesis (STM) was adapted to H. pylori. The Mongolian gerbil (Meriones unguiculatus) was used as model system to screen a set of 960 STM mutants. This resulted in 47 H. pylori genes, assigned to 9 different functional categories, representing a set of biological functions absolutely essential for gastric colonization, as verified and quantified for many mutants by competition experiments. Identification of previously known colonization factors, such as the urease and motility functions validated this method, but also novel and several hypothetical genes were found. Interestingly, a secreted collagenase, encoded by hp0169, could be identified and functionally verified as a new essential virulence factor for H. pylori stomach colonization. Furthermore, comB4, encoding a putative ATPase being part of a DNA transformation-associated type IV transport system of H. pylori was found to be absolutely essential for colonization, but natural transformation competence was apparently not the essential function. Thus, this first systematic STM application identified a set of previously unknown H. pylori colonization factors and may help to potentiate the development of novel therapies against gastric Helicobacter infections.
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