ArticleBiophysical journal2003
The effects of intense submicrosecond electrical pulses on cells.
Article in Biophysical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
50 citing papers in PubMed, 237 citations in OpenAlex.
- OpenPore: A low-cost, portable, battery-powered exponential decay pulse generator for electroporation.HardwareX · 2026Article
- Resolving nanosecond kinetics of the optical membrane potential in pulsed electric fields.Bioelectrochemistry (Amsterdam, Netherlands) · 2026Article
- Spatiotemporal analysis of Escherichia coli membrane permeabilization and uptake kinetics induced by a single microbubble cavitation event.Scientific reports · 2025Article
- Comparative study of nanosecond and microsecond pulsed field ablation for pulmonary vein isolation in preclinical models.Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology · 2025Article
- Pulsed Field Ablation for the Treatment of Atrial Fibrillation: A Review and a Look into its Future.The heart surgery forum · 2024Article
- Efficacy of metformin and electrical pulses in breast cancer MDA-MB-231 cells.Exploration of targeted anti-tumor therapy · 2024Article
- Influence of electric potential-induced by atmospheric pressure plasma on cell response.Scientific reports · 2023Article
- How to alleviate cardiac injury from electric shocks at the cellular level.Frontiers in cardiovascular medicine · 2022Article
- Does the shape of the electric pulse matter in electroporation?Frontiers in oncology · 2022Review
- Ultralong-Time Recovery and Low-Voltage Electroporation for Biological Cell Monitoring Enabled by a Microsized Multipulse Framework.ACS omega · 2021Article
- Irreversible Electroporation Enhanced by Radiofrequency Ablation: An In Vitro and Computational Study in a 3D Liver Tumor Model.Annals of biomedical engineering · 2021Article
- Characterization of Cell Membrane Permeability In Vitro Part I: Transport Behavior Induced by Single-Pulse Electric Fields.Technology in cancer research & treatment · 2018Article
- Experimental Nanopulse Ablation of Multiple Membrane Parasite on Ex Vivo Hydatid Cyst.BioMed research international · 2018Article
- Harnessing the hygroscopic and biofluorescent behaviors of genetically tractable microbial cells to design biohybrid wearables.Science advances · 2017Article
- Effect of Cooling On Cell Volume and Viability After Nanoelectroporation.The Journal of membrane biology · 2017Article
- A new mechanism for efficient hydrocarbon electro-extraction fromBiotechnology for biofuels · 2017Article
- Nanosecond electric pulses differentially affect inward and outward currents in patch clamped adrenal chromaffin cells.PloS one · 2017Article
- The cytotoxic synergy of nanosecond electric pulses and low temperature leads to apoptosis.Scientific reports · 2016Article
- Effects of Cisplatin Electrochemotherapy on Human Neuroblastoma Cells.The Journal of membrane biology · 2016Article
- [Mechanism of ablation with nanosecond pulsed electric field].Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A simple electrical model for living cells predicts an increasing probability for electric field interactions with intracellular substructures of both prokaryotic and eukaryotic cells when the electric pulse duration is reduced into the sub-microsecond range. The validity of this hypothesis was verified experimentally by applying electrical pulses (durations 100 micros-60 ns, electric field intensities 3-150 kV/cm) to Jurkat cells suspended in physiologic buffer containing propidium iodide. Effects on Jurkat cells were assessed by means of temporally resolved fluorescence and light microscopy. For the longest applied pulses, immediate uptake of propidium iodide occurred consistent with electroporation as the cause of increased surface membrane permeability. For nanosecond pulses, more delayed propidium iodide uptake occurred with significantly later uptake of propidium iodide occurring after 60 ns pulses compared to 300 ns pulses. Cellular swelling occurred rapidly following 300 ns pulses, but was minimal following 60 ns pulses. These data indicate that submicrosecond pulses achieve temporally distinct effects on living cells compared to microsecond pulses. The longer pulses result in rapid permeability changes in the surface membrane that are relatively homogeneous across the cell population, consistent with electroporation, while shorter pulses cause surface membrane permeability changes that are temporally delayed and heterogeneous in their magnitude.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.