ArticleBiophysical journal2003
Mapping mechanical strain of an endogenous cytoskeletal network in living endothelial cells.
Article in Biophysical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
43 citing papers in PubMed, 141 citations in OpenAlex.
- Structural response of microtubule and actin cytoskeletons to direct intracellular load.The Journal of cell biology · 2025Article
- Endothelial cell elongation and alignment in response to shear stress requires acetylation of microtubules.Frontiers in physiology · 2024Article
- Viscoelasticity, Like Forces, Plays a Role in Mechanotransduction.Frontiers in cell and developmental biology · 2022Review
- Mechanisms underlying unidirectional laminar shear stress-mediated Nrf2 activation in endothelial cells: Amplification of low shear stress signaling by primary cilia.Redox biology · 2021Review
- Editorial: Understanding molecular interactions that underpin vascular mechanobiology.APL bioengineering · 2021Article
- Bone fracture healing: perspectives according to molecular basis.Journal of bone and mineral metabolism · 2021Review
- Biofabrication Strategies and Engineered In Vitro Systems for Vascular Mechanobiology.Advanced healthcare materials · 2020Review
- Adjustable viscoelasticity allows for efficient collective cell migration.Seminars in cell & developmental biology · 2019Review
- Perioperative implication of the endothelial glycocalyx.Korean journal of anesthesiology · 2018Review
- The nesprin-cytoskeleton interface probed directly on single nuclei is a mechanically rich system.Nucleus (Austin, Tex.) · 2017Article
- Design of a microfluidic device to quantify dynamic intra-nuclear deformation during cell migration through confining environments.Integrative biology : quantitative biosciences from nano to macro · 2015Article
- Shear stress induces a longitudinal Ca(2+) wave via autocrine activation of P2Y1 purinergic signalling in rat atrial myocytes.The Journal of physiology · 2015Article
- Tendon mechanobiology: Current knowledge and future research opportunities.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2015Review
- Polarized actin structural dynamics in response to cyclic uniaxial stretch.Cellular and molecular bioengineering · 2015Article
- High Pulsatility Flow Induces Acute Endothelial Inflammation through Overpolarizing Cells to Activate NF-κB.Cardiovascular engineering and technology · 2013Article
- Strain-induced tissue growth laws: applications to embryonic cardiovascular development.Journal of applied mechanical engineering · 2013Article
- Cell mechanosensitivity: mechanical properties and interaction with gravitational field.BioMed research international · 2013Review
- Fellow travellers: emergent properties of collective cell migration.EMBO reports · 2012Review
- The functions of the nuclear envelope in mediating the molecular crosstalk between the nucleus and the cytoplasm.Current opinion in cell biology · 2012Review
- Zyxin is involved in regulation of mechanotransduction in arteriole smooth muscle cells.Frontiers in physiology · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
A central aspect of cellular mechanochemical signaling is a change of cytoskeletal tension upon the imposition of exogenous forces. Here we report measurements of the spatiotemporal distribution of mechanical strain in the intermediate filament cytoskeleton of endothelial cells computed from the relative displacement of endogenous green fluorescent protein (GFP)-vimentin before and after onset of shear stress. Quantitative image analysis permitted computation of the principal values and orientations of Lagrangian strain from 3-D high-resolution fluorescence intensity distributions that described intermediate filament positions. Spatially localized peaks in intermediate filament strain were repositioned after onset of shear stress. The orientation of principal strain indicated that mechanical stretching was induced across cell boundaries. This novel approach for intracellular strain mapping using an endogenous reporter demonstrates force transfer from the lumenal surface throughout the cell.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.