ArticleBiophysical journal2003
Properties of the demarcation membrane system in living rat megakaryocytes.
Article in Biophysical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- A theory of rapid evolutionary change explaining the de novo appearance of megakaryocytes and platelets in mammals.Journal of cell science · 2022Article
- Review
- Development of a P2X1-eYFP receptor knock-in mouse to track receptors in real time.Purinergic signalling · 2019Article
- Imaging platelet biogenesis in vivo.Research and practice in thrombosis and haemostasis · 2018Review
- Hyaluronan Depolymerization by Megakaryocyte Hyaluronidase-2 Is Required for Thrombopoiesis.The American journal of pathology · 2016Article
- Article
- Detachment of surface membrane invagination systems by cationic amphiphilic drugs.Scientific reports · 2016Article
- FlnA binding to PACSIN2 F-BAR domain regulates membrane tubulation in megakaryocytes and platelets.Blood · 2015Article
- Dynamin 2-dependent endocytosis is required for normal megakaryocyte development in mice.Blood · 2015Article
- Physiological significance of delayed rectifier K(+) channels (Kv1.3) expressed in T lymphocytes and their pathological significance in chronic kidney disease.The journal of physiological sciences : JPS · 2015Review
- Systems modeling of Ca(2+) homeostasis and mobilization in platelets mediated by IP3 and store-operated Ca(2+) entry.Biophysical journal · 2014Article
- Distinct functional effects for dynamin 3 during megakaryocytopoiesis.Stem cells and development · 2011Article
- Article
- Myosin-II inhibition and soft 2D matrix maximize multinucleation and cellular projections typical of platelet-producing megakaryocytes.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Compensatory thrombopoietin production from the liver and bone marrow stimulates thrombopoiesis of living rat megakaryocytes in chronic renal failure.Nephron extra · 2011Article
- Thrombocytogenesis by megakaryocyte; Interpretation by protoplatelet hypothesis.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2011Review
- Direct voltage control of endogenous lysophosphatidic acid G-protein-coupled receptors in Xenopus oocytes.The Journal of physiology · 2010Article
- Kv1.3 is the exclusive voltage-gated K+ channel of platelets and megakaryocytes: roles in membrane potential, Ca2+ signalling and platelet count.The Journal of physiology · 2010Article
- Platelet production by megakaryocytes: protoplatelet theory justifies cytoplasmic fragmentation model.International journal of hematology · 2008Review
- Molecular and electrophysiological characterization of transient receptor potential ion channels in the primary murine megakaryocyte.The Journal of physiology · 2006Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The demarcation membrane system (DMS) is the precursor of platelet cell membranes yet little is known of its properties in living megakaryocytes. Using confocal microscopy, we now demonstrate that demarcation membranes in freshly isolated rat marrow megakaryocytes are rapidly stained by styryl membrane indicators such as di-8-ANEPPS and FM 2-10, confirming that they are invaginations of the plasma membrane and readily accessible from the extracellular space. Two-photon excitation of an extracellular indicator displayed the extensive nature of the channels formed by the DMS throughout the extranuclear volume. Under whole-cell patch clamp, the DMS is electrophysiologically contiguous with the peripheral plasma membrane such that a single capacitative component can account for the biophysical properties of all surface-connected membranes in the majority of recordings. Megakaryocyte capacitances were in the range of 64-694 pF, equivalent to 500-5500 platelets (mean value 1850). Based upon calculations for a spherical geometry, the DMS results in a 4- to 14-fold (average 8.1-fold) increase in specific membrane capacitance expressed per unit spherical surface area. This indicates a level of plasma membrane invagination comparable with mammalian skeletal muscle. Whole-cell capacitance measurements and confocal imaging of membrane-impermeant fluorescent indicators therefore represent novel approaches to monitor the DMS during megakaryocytopoiesis and thrombopoiesis.
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