ArticleBiophysical journal2003
Diffusional mobility of parvalbumin in spiny dendrites of cerebellar Purkinje neurons quantified by fluorescence recovery after photobleaching.
Article in Biophysical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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Who cites it
34 citing papers in PubMed, 75 citations in OpenAlex.
- Physiology of intracellular calcium buffering.Physiological reviews · 2023Review
- Expanding the applicability of multiphoton fluorescence recovery after photobleaching by incorporating shear stress in laminar flow.Journal of biomedical optics · 2023Article
- Computational modeling predicts ephemeral acidic microdomains in the glutamatergic synaptic cleft.Biophysical journal · 2021Article
- Cytosolic CaCold Spring Harbor perspectives in biology · 2020Review
- Complex spike clusters and false-positive rejection in a cerebellar supervised learning rule.The Journal of physiology · 2019Article
- Tonotopy in calcium homeostasis and vulnerability of cochlear hair cells.Hearing research · 2019Review
- Variations in CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Padé Approximation of a Stationary Single-Channel CaBiophysical journal · 2016Article
- A use-dependent increase in release sites drives facilitation at calretinin-deficient cerebellar parallel-fiber synapses.Frontiers in cellular neuroscience · 2015Article
- Buffer mobility and the regulation of neuronal calcium domains.Frontiers in cellular neuroscience · 2015Review
- Estimation of presynaptic calcium currents and endogenous calcium buffers at the frog neuromuscular junction with two different calcium fluorescent dyes.Frontiers in synaptic neuroscience · 2014Article
- Sources of parvalbumine innervation of the thalamic center of the tectofugal visual pathway in turtles.Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections · 2013Article
- Restricted diffusion of calretinin in cerebellar granule cell dendrites implies Ca²⁺-dependent interactions via its EF-hand 5 domain.The Journal of physiology · 2013Article
- Diffusion and extrusion shape standing calcium gradients during ongoing parallel fiber activity in dendrites of Purkinje neurons.Cerebellum (London, England) · 2012Article
- Nanodomain coupling between Ca²⁺ channels and sensors of exocytosis at fast mammalian synapses.Nature reviews. Neuroscience · 2011Review
- How the 'slow' Ca(2+) buffer parvalbumin affects transmitter release in nanodomain-coupling regimes.Nature neuroscience · 2011Article
- Cytosolic Ca2+ buffers.Cold Spring Harbor perspectives in biology · 2010Review
- Compensatory regulation of Cav2.1 Ca2+ channels in cerebellar Purkinje neurons lacking parvalbumin and calbindin D-28k.Journal of neurophysiology · 2010Article
- Spine neck geometry determines spino-dendritic cross-talk in the presence of mobile endogenous calcium binding proteins.Journal of computational neuroscience · 2009Article
- Modeling of the modulation by buffers of Ca2+ release through clusters of IP3 receptors.Biophysical journal · 2009Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ca(2+)-binding proteins (CaBPs) represent key factors for the modulation of cellular Ca(2+) dynamics. Especially in thin extensions of nerve cells, Ca(2+) binding and buffered diffusion of Ca(2+) by CaBPs is assumed to effectively control the spatio-temporal extend of Ca(2+) signals. However, no quantitative data about the mobility of specific CaBPs in the neuronal cytosol are available. We quantified the diffusion of the endogenous CaPB parvalbumin (PV) in spiny dendrites of cerebellar Purkinje neurons with two-photon fluorescence recovery after photobleaching. Fluorescently labeled PV diffused readily between spines and dendrites with a median time constant of 49 ms (37-61 ms, interquartile range). Based on published data on spine geometry, this value corresponds to an apparent diffusion coefficient of 43 microm(2) s(-1) (34-56 microm(2) s(-1)). The absence of large or immobile binding partners for PV was confirmed in PV null-mutant mice. Our data validate the common but so far unproven assumption that PV is highly mobile in neurons and will facilitate simulations of neuronal Ca(2+) buffering. Our experimental approach represents a versatile tool for quantifying the mobility of proteins in neuronal dendrites.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.