ArticleMolecular and cellular biology2003
Abolition of cyclin-dependent kinase inhibitor p16Ink4a and p21Cip1/Waf1 functions permits Ras-induced anchorage-independent growth in telomerase-immortalized human fibroblasts.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 69 citations in OpenAlex.
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- Cell-cycle-regulated activation of Akt kinase by phosphorylation at its carboxyl terminus.Nature · 2014Article
- How to measure RNA expression in rare senescent cells expressing any specific protein such as p16Ink4a.Aging · 2013Article
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- Proteomic profiling of Myc-associated proteins.Cell cycle (Georgetown, Tex.) · 2010Article
- The miR-17-92 cluster of microRNAs confers tumorigenicity by inhibiting oncogene-induced senescence.Cancer research · 2010Article
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- A structure-guided mutational analysis of simian virus 40 large T antigen: identification of surface residues required for viral replication and transformation.Journal of virology · 2009Article
- Mitochondrial dysfunction contributes to oncogene-induced senescence.Molecular and cellular biology · 2009Article
- Cdh1 regulates cell cycle through modulating the claspin/Chk1 and the Rb/E2F1 pathways.Molecular biology of the cell · 2009Article
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- Ras oncogenes: split personalities.Nature reviews. Molecular cell biology · 2008Review
- Hepatitis B virus X protein overcomes oncogenic RAS-induced senescence in human immortalized cells.Cancer science · 2007Article
- SIRT2 regulates adipocyte differentiation through FoxO1 acetylation/deacetylation.Cell metabolism · 2007Article
- CDK4 and CDK6 delay senescence by kinase-dependent and p16INK4a-independent mechanisms.Molecular and cellular biology · 2007Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Human cells are more resistant to both immortalization and malignant transformation than rodent cells. Recent studies have established the basic genetic requirements for the transformation of human cells, but much of this work relied on the expression of transforming proteins derived from DNA tumor viruses. We constructed an isogenic panel of human fibroblast cell lines using a combination of gene targeting and ectopic expression of dominantly acting mutants of cellular genes. Abolition of p21(Cip1/Waf1) and p16(Ink4a) functions prevented oncogenically activated Ras from inducing growth arrest and was sufficient for limited anchorage-independent growth but not tumorigenesis. Deletion of the tumor suppressor p53 combined with abolition of p16(Ink4a) function failed to mimic the introduction of simian virus 40 large T antigen, indicating that large T antigen may target additional cellular functions. Ha-Ras and Myc cooperated only to a limited extent, but in the absence of Ras, Myc cooperated strongly with the simian virus 40 small t antigen to elicit aggressive anchorage-independent growth. The experiments reported here further define specific components of human transformation pathways.
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