ArticleJournal of virology2003
A synthetic E7 gene of human papillomavirus type 16 that yields enhanced expression of the protein in mammalian cells and is useful for DNA immunization studies.
Article in Journal of virology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 124 citations in OpenAlex.
- Mutation Profiles, Glycosylation Site Distribution and Codon Usage Bias of Human Papillomavirus Type 16.Viruses · 2021Article
- Soluble Expression and Catalytic Properties of Codon-Optimized Recombinant Bromelain from MD2 Pineapple in Escherichia coli.The protein journal · 2021Article
- Long-Term Antitumor CD8Cancers · 2021Article
- The C-Terminal Domain of NefVaccines · 2021Article
- The Intracellular Delivery Of Anti-HPV16 E7 scFvs Through Engineered Extracellular Vesicles Inhibits The Proliferation Of HPV-Infected Cells.International journal of nanomedicine · 2019Article
- Therapeutic DNA Vaccines for Human Papillomavirus and Associated Diseases.Human gene therapy · 2018Article
- Preparation of virus-like particles for porcine circovirus type 2 by YeastFab Assembly.Virus genes · 2018Article
- Telomere Dynamics in Immune Senescence and Exhaustion Triggered by Chronic Viral Infection.Viruses · 2017Review
- Cancer, Warts, or Asymptomatic Infections: Clinical Presentation Matches Codon Usage Preferences in Human Papillomaviruses.Genome biology and evolution · 2015Article
- Papillomaviruses: Viral evolution, cancer and evolutionary medicine.Evolution, medicine, and public health · 2015Review
- Effects of the fusion design and immunization route on the immunogenicity of Ag85A-Mtb32 in adenoviral vectored tuberculosis vaccine.Human vaccines & immunotherapeutics · 2015Article
- Article
- Determinants of baseline seroreactivity to human papillomavirus type 16 in the Ludwig-McGill cohort study.BMC infectious diseases · 2014Article
- Imaging of genetically engineered T cells by PET using gold nanoparticles complexed to Copper-64.Integrative biology : quantitative biosciences from nano to macro · 2013Article
- A multiagent filovirus DNA vaccine delivered by intramuscular electroporation completely protects mice from ebola and Marburg virus challenge.Human vaccines & immunotherapeutics · 2012Article
- Virus-Like Particles Harboring CCL19, IL-2 and HPV16 E7 Elicit Protective T Cell Responses in HLA-A2 Transgenic Mice.The open virology journal · 2012Article
- Preventive and Therapeutic Vaccines against Human Papillomaviruses Associated Cervical Cancers.Iranian journal of basic medical sciences · 2012Article
- Characterization and whole genome analysis of human papillomavirus type 16 e1-1374^63nt variants.PloS one · 2012Article
- A DNA vaccine for venezuelan equine encephalitis virus delivered by intramuscular electroporation elicits high levels of neutralizing antibodies in multiple animal models and provides protective immunity to mice and nonhuman primates.Clinical and vaccine immunology : CVI · 2011Article
- Therapeutic HPV DNA vaccines.Immunologic research · 2010Review
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A synthetic E7 gene of human papillomavirus (HPV) type 16 was generated that consists entirely of preferred human codons. Expression analysis of the synthetic E7 gene in human and animal cells showed levels of E7 protein 20- to 100-fold higher than those obtained with wild-type E7. Enhanced expression of E7 protein resulted from highly efficient translation, as well as increased stability of the E7 mRNA due to its codon optimization. Higher levels of E7 protein in cells transfected with synthetic E7 correlated with significant loss of cell viability in various human cell lines. In contrast, lower E7 protein expression driven by the wild-type gene resulted in a slight induction of cell proliferation. Furthermore, mice inoculated with plasmids expressing the synthetic E7 gene produced significantly higher levels of E7 antibodies than littermates injected with wild-type E7, suggesting that synthetic E7 may be useful for DNA immunization studies and the development of genetic vaccines against HPV-16. In view of these results, we hypothesize that HPVs may have retained a pattern of G + C content and codon usage distinct from that of their host cells in response to selective pressure. Thus, the nonhuman codon bias may have been conserved by HPVs to prevent compromising viability of the host cells by excessive viral early protein expression, as well as to evade the immune system.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.