ArticleJournal of virology2003
Switching species tropism: an effective way to manipulate the feline coronavirus genome.
Article in Journal of virology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 102 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
102 citing papers in PubMed, 151 citations in OpenAlex.
- Glycyrrhiza polysaccharide inhibits feline infectious peritonitis virus infection screened by a recombinant virus expressing eGFP protein.BMC veterinary research · 2026Article
- Engineering a mouse-adapted SADS-CoV and establishing a neonatal mouse model to study its infection.mBio · 2026Article
- Article
- Development of a yeast-based CRISPR genome editing system for feline coronavirus.Frontiers in microbiology · 2025Article
- Caution urged for work with newly constructed human coronavirus.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Development and characterization of reverse genetics systems of feline infectious peritonitis virus for antiviral research.Veterinary research · 2024Article
- Reverse Genetics Systems for Emerging and Re-Emerging Swine Coronaviruses and Applications.Viruses · 2023Review
- A One Health Perspective on Canine Coronavirus: A Wolf in Sheep's Clothing?Microorganisms · 2023Review
- Natural selection differences detected in key protein domains between non-pathogenic and pathogenic Feline Coronavirus phenotypes.bioRxiv : the preprint server for biology · 2023Article
- Natural selection differences detected in key protein domains between non-pathogenic and pathogenic feline coronavirus phenotypes.Virus evolution · 2023Article
- Reverse genetics systems for SARS-CoV-2.Journal of medical virology · 2022Review
- Article
- Review
- Article
- Diversity of Coronaviruses with Particular Attention to the Interspecies Transmission of SARS-CoV-2.Animals : an open access journal from MDPI · 2022Review
- Porcine Deltacoronaviruses: Origin, Evolution, Cross-Species Transmission and Zoonotic Potential.Pathogens (Basel, Switzerland) · 2022Review
- Cell Entry of Animal Coronaviruses.Viruses · 2021Review
- Clinical and molecular aspects of veterinary coronaviruses.Virus research · 2021Review
- Article
- Spike glycoproteins: Their significance for corona viruses and receptor binding activities for pathogenesis and viral survival.Microbial pathogenesis · 2021Review
42 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Feline infectious peritonitis virus (FIPV), a coronavirus, is the causative agent of an invariably lethal infection in cats. Like other coronaviruses, FIPV contains an extremely large positive-strand RNA genome of ca. 30 kb. We describe here the development and use of a reverse genetics strategy for FIPV based on targeted RNA recombination that is analogous to what has been described for the mouse hepatitis virus (MHV) (L. Kuo et al., J. Virol. 74:1393-1406, 2000). In this two-step process, we first constructed by targeted recombination a mutant of FIPV, designated mFIPV, in which the ectodomain of the spike glycoprotein was replaced by that of MHV. This switch allowed for the selection of the recombinant virus in murine cells: mFIPV grows to high titers in these cells but has lost the ability to grow in feline cells. In a second, reverse process, mFIPV was used as the recipient, and the reintroduction of the FIPV spike now allowed for selection of candidate recombinants by their regained ability to grow in feline cells. In this fashion, we reconstructed a wild-type recombinant virus (r-wtFIPV) and generated a directed mutant FIPV in which the initiation codon of the nonstructural gene 7b had been disrupted (FIPV Delta 7b). The r-wtFIPV was indistinguishable from its parental virus FIPV 79-1146 not only for its growth characteristics in tissue culture but also in cats, exhibiting a highly lethal phenotype. FIPV Delta 7b had lost the expression of its 7b gene but grew unimpaired in cell culture, confirming that the 7b glycoprotein is not required in vitro. We establish the second targeted RNA recombination system for coronaviruses and provide a powerful tool for the genetic engineering of the FIPV genome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.