ArticleJournal of virology2003
Silencing of the baculovirus Op-iap3 gene by RNA interference reveals that it is required for prevention of apoptosis during Orgyia pseudotsugata M nucleopolyhedrovirus infection of Ld652Y cells.
Article in Journal of virology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 61 citations in OpenAlex.
- RNAi turns 25:contributions and challenges in insect science.Frontiers in insect science · 2023Review
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- BmNPV p35 Reduces the Accumulation of Virus-Derived siRNAs and Hinders the Function of siRNAs to Facilitate Viral Infection.Frontiers in immunology · 2022Article
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- Characterization and functional assay of apsup (Lyxy105) from Lymantria xylina multiple nucleopolyhedrovirus (LyxyMNPV).Virus genes · 2018Article
- The Baculovirus Antiapoptotic p35 Protein Functions as an Inhibitor of the Host RNA Interference Antiviral Response.Journal of virology · 2015Article
- Novel apoptosis suppressor Apsup from the baculovirus Lymantria dispar multiple nucleopolyhedrovirus precludes apoptosis by preventing proteolytic processing of initiator caspase Dronc.Journal of virology · 2013Article
- Enhanced recombinant protein production and differential expression of molecular chaperones in sf-caspase-1-repressed stable cells after baculovirus infection.BMC biotechnology · 2012Article
- Baculovirus infection induces a DNA damage response that is required for efficient viral replication.Journal of virology · 2011Article
- Induction of reaper ortholog mx in mosquito midgut cells following baculovirus infection.Cell death and differentiation · 2011Article
- Host insect inhibitor-of-apoptosis SfIAP functionally replaces baculovirus IAP but is differentially regulated by Its N-terminal leader.Journal of virology · 2010Article
- Identification and functional analysis of the putative anti-apoptotic gene iap4 of Spodoptera litura nucleopolyhedrovirus.Virus genes · 2010Article
- Functional analysis of the inhibitor of apoptosis genes in Antheraea pernyi nucleopolyhedrovirus.Journal of microbiology (Seoul, Korea) · 2010Article
- Baculovirus DNA replication-specific expression factors trigger apoptosis and shutoff of host protein synthesis during infection.Journal of virology · 2009Article
- Transactivator IE1 is required for baculovirus early replication events that trigger apoptosis in permissive and nonpermissive cells.Journal of virology · 2009Article
- Studies of the silencing of baculovirus DNA binding protein.Journal of virology · 2007Article
- Article
- Expression of baculovirus late and very late genes depends on LEF-4, a component of the viral RNA polymerase whose guanyltransferase function is essential.Journal of virology · 2006Article
- RNA interference: its use as antiviral therapy.Handbook of experimental pharmacology · 2006Review
- Functional analysis of the inhibitor of apoptosis (iap) gene carried by the entomopoxvirus of Amsacta moorei.Journal of virology · 2005Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The Op-iap3 gene from the baculovirus Orgyia pseudotsugata M nucleopolyhedrovirus (OpMNPV) inhibits apoptosis induced by a mutant of Autographa californica MNPV (AcMNPV) that lacks the antiapoptotic gene p35, as well as apoptosis induced by a wide range of other stimuli in both mammalian and insect cells. However, the role of Op-iap3 during OpMNPV infection has not been previously examined. To determine the function of the Op-IAP3 protein during OpMNPV infection, we used RNA interference (RNAi) to silence Op-iap3 expression during OpMNPV infection of Ld652Y cells. Infected cells treated with Op-iap3 double-stranded RNA (dsRNA) did not accumulate detectable Op-iap3 mRNA, confirming that the Op-iap3 gene was effectively silenced. Op-IAP3 protein was found to be a component of the budded virion; however, in OpMNPV-infected cells treated with Op-iap3 dsRNA, the Op-IAP3 protein that was introduced by the inoculum virus decreased to almost undetectable levels by 12 h after dsRNA addition. Apoptosis was observed in infected cells treated with Op-iap3 dsRNA beginning at 12 h, and by 48 h, almost all of the cells had undergone apoptosis. These results show for the first time that Op-IAP3 is necessary to prevent apoptosis during OpMNPV infection. In addition, our results demonstrate that the RNAi technique can be an effective tool for studying baculovirus gene function.
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