ArticleGenetics2003
Multiple translational control sequences in the 5' leader of the chloroplast psbC mRNA interact with nuclear gene products in Chlamydomonas reinhardtii.
Article in Genetics, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 26 citations in OpenAlex.
- LPA2 protein is involved in photosystem II assembly in Chlamydomonas reinhardtii.The Plant journal : for cell and molecular biology · 2021Article
- Pas de Trois: An Overview of Penta-, Tetra-, and Octo-Tricopeptide Repeat Proteins FromFrontiers in plant science · 2021Review
- The RNA Structure of cis-acting Translational Elements of the Chloroplast psbC mRNA in Chlamydomonas reinhardtii.Frontiers in plant science · 2016Article
- How to build functional thylakoid membranes: from plastid transcription to protein complex assembly.Planta · 2013Review
- Light-dependent attenuation of phycoerythrin gene expression reveals convergent evolution of green light sensing in cyanobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Article
- Regulatory sequences of orthologous petD chloroplast mRNAs are highly specific among Chlamydomonas species.Plant molecular biology · 2006Article
- Translation of chloroplast psbD mRNA in Chlamydomonas is controlled by a secondary RNA structure blocking the AUG start codon.Nucleic acids research · 2006Article
- RNA-protein complexes that form in the spinach chloroplast atpI 5' untranslated region can be divided into two subcomplexes, each comprised of unique cis-elements and trans-factors.Current genetics · 2005Article
- Specific roles of 5' RNA secondary structures in stabilizing transcripts in chloroplasts.Nucleic acids research · 2005Article
- Chlamydomonas reinhardtii at the crossroads of genomics.Eukaryotic cell · 2003Review
- Chloroplast translation regulation.Photosynthesis researchReview
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Translation of the chloroplast psbC mRNA in the unicellular eukaryotic alga Chlamydomonas reinhardtii is controlled by interactions between its 547-base 5' untranslated region and the products of the nuclear loci TBC1, TBC2, and possibly TBC3. In this study, a series of site-directed mutations in this region was generated and the ability of these constructs to drive expression of a reporter gene was assayed in chloroplast transformants that are wild type or mutant at these nuclear loci. Two regions located in the middle of the 5' leader and near the initiation codon are important for translation. Other deletions still allow for partial expression of the reporter gene in the wild-type background. Regions with target sites for TBC1 and TBC2 were identified by estimating the residual translation activity in the respective mutant backgrounds. TBC1 targets include mostly the central part of the leader and the translation initiation region whereas the only detected TBC2 targets are in the 3' part. The 5'-most 93 nt of the leader are required for wild-type levels of transcription and/or mRNA stabilization. The results indicate that TBC1 and TBC2 function independently and further support the possibility that TBC1 acts together with TBC3.
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